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Comparative microbiome analysis of pesticide contaminated and pristine agricultural soil of hot arid Rajasthan

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Title Comparative microbiome analysis of pesticide contaminated and pristine agricultural soil of hot arid Rajasthan
Not Available
 
Creator Pancholy Anjly
S.K. Singh
 
Subject Microbiome
16S rRNA
pesticides
arid soil
 
Description Not Available
Comparative microbial community structure analysis of agricultural soil located in
hot, western Rajasthan with long term history of chemical pesticides vis-à-vis
pristine soil has been attempted using the technique of PCR-RFLP. Pesticide contaminated (24 nos.) and pristine (10 nos.) soil samples were collected from
different locations of the vegetable growing agricultural fields. Metagenomic
DNA was extracted each from pooled-contaminated and pooled-pristine soils and
amplified with universal 16S rRNA gene primers. Amplification products were
cloned in to E. coli DH5α cells to obtain 78 and 39 16S rDNA clones from
contaminated and pristine soils respectively. The clones were subjected to RFLP
analysis using three restriction enzymes (AluI, AvaII and MspI) followed by
phylogenetic tree construction. The studies revealed differences in microbial
community structures of both types of arid, agricultural soils. We also detected the
presence of pesticide-degrading naphthalene dioxygenase gene (nahA) controlling
the initial step of the degradation process in contaminated soil.
Not Available
 
Date 2021-07-23T09:48:22Z
2021-07-23T09:48:22Z
2020-01-01
 
Type Research Paper
 
Identifier Anjly Pancholy and Singh, S.K. 2020. Comparative Microbiome Analysis of PesticideContaminated and Pristine Agricultural Soils of Hot Arid Rajasthan. Int.J.Curr.Microbiol.App.Sci. 9(11): 1962-1970. doi: https://doi.org/10.20546/ijcmas.2020.911.233
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/49593
 
Language English
 
Relation Not Available;
 
Publisher Excellent publishers