Record Details

Effect of pretreatment of explants and in vitro culture media in controlling infection and browning in Kusum [Schleichera oleosa (Lour.) Oken]

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Effect of pretreatment of explants and in vitro culture media in controlling infection and browning in Kusum [Schleichera oleosa (Lour.) Oken]
Not Available
 
Creator Pradip Kumar Sarkar
Animesh Sinha
B.P. Bhatt
Rajneesh Kumar
Atanu Sarkar
Sushit Banerjee
Jyotirmoy Ghosh
Ruby S Kujur
Nitin Kulkarni
 
Subject Kusum tree
in vitro culture
browning
infection
shoot initiation
 
Description Not Available
The Kusum tree [Schleichera oleosa (Lour.) Oken] is being exploited in India mainly for lac production but also being known for its multiple uses like ethno-medicinal value, edible-fruits, seed oil as medicinal and biodiesel uses. But, very little attention has been given in the direction of tree improvement of this species. Moreover, in vitro propagation of woody species poses problems due to high contamination rate and exudation of phenolics. Hence, to overcome such problems, an experiment was conducted to study the effect of pretreatment of explants and in vitro culture media in controlling infection and browning in Kusum. Thus, for disinfection of explants of Kusum, nine different treatments as surface sterilants were considered. While, for controlling browning, the ten treatments consisting of different additives including antioxidants were prepared for the study. The results revealed that, the nodal explants treated with HgCl2 (0.1%) for 7 minutes and NaOCl (7%) for 5 minutes were found to be the best treatments in controlling infection (i.e., 83.33% and 70.91% respectively) in the culture media with respect to maximum survival of explants on Day 30 (i.e., 78.37% and 73.06%, respectively). On the other hand, Ascorbic acid (ranges from 100 mg L-1 to 200 mg L-1) added in MS (Murashige and Skoog) media had played a significant role and had effectively controlled the browning when compared with control. The best season for in vitro culture of Kusum was found to be the summer season mainly during the months from February to May end.
Not Available
 
Date 2021-08-07T06:12:08Z
2021-08-07T06:12:08Z
2019-12-01
 
Type Research Paper
 
Identifier Not Available
2349-8528
http://krishi.icar.gov.in/jspui/handle/123456789/53533
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Chemical Studies