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Effect of High-Temperature Stress on Ascorbate–Glutathione Cycle in Maize

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Title Effect of High-Temperature Stress on Ascorbate–Glutathione Cycle in Maize
Not Available
 
Creator S. K. Yadav
 
Subject Antioxidative enzymes
Heat stress
Lipid peroxidation
Non-enzymatic antioxidants
 
Description Not Available
High-temperature stress induces cellular changes leading to over-production of highly reactive oxygen species
(ROS) which damage macromolecules and cell organelles, eventually resulting in cell death. Antioxidative metabolism in
plants comprising of enzymatic and non-enzymatic antioxidants imparts tolerance by scavenging or detoxification of excess
ROS. We investigated the response of major H2O2-detoxifying system, the AsA–GSH cycle in four genotypes of maize
differing in heat sensitivity. Stress was imposed by staggered sowing so that one set of plants faced high-temperature stress at
their anthesis-silking stage. The concentrations of H2O2 increased across the genotypes by high temperature; however, the
increase was lesser in tolerant genotypes: NSJ 189 and NSJ 221. High-temperature stress led to an increase in the level of GSH
and GSSG in all the genotypes, whereas GSH/GSSG decreased in sensitive genotypes: PSRJ 13099 and RJR 270. The
glutathione S-transferase activity increased significantly under heat stress. APX, MDHAR and DHAR activities decreased
under heat stress in the sensitive group. Under high temperature, GR activity remained unchanged in sensitive genotypes,
while it increased significantly in tolerant genotypes. Ascorbate levels increased in tolerant genotypes, while a decline was
observed in sensitive genotypes. Isoforms of APX showed some new bands in tolerant genotypes as well as higher intensity of
the existing ones as compared to sensitive genotypes under stress conditions. Isoforms of GR did not show any genotypic
differences under heat stress. Findings emphasized the importance and complexity of the AsA–GSH system in fine-tuning the
redox metabolism under heat stress in maize. The study also suggested that the antioxidative enzymes of AsA–GSH cycle play
a key role in sustaining the ROS homeostasis in cells, thus minimizing the potential toxic effects of ROS.
Not Available
 
Date 2021-08-17T06:32:19Z
2021-08-17T06:32:19Z
2020-06-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/57471
 
Language English
 
Relation Not Available;
 
Publisher Cross Mark