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Egg yolk powder an alternative to fresh-egg yolk for buffalo (Bubalus bubalis) semen cryopreservation

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Title Egg yolk powder an alternative to fresh-egg yolk for buffalo (Bubalus bubalis) semen cryopreservation
Not Available
 
Creator MAHAK SINGH
NC BARIK
SUBRATA KUMAR GHOSH
J K PRASAD
 
Subject Buffalo semen
Cryopreservation
Egg yolk powder
Semen
Sperm
 
Description Not Available
The present work was carried out to evaluate the effect of commercial egg yolk (EY) powder as an alternative
to fresh EY on buffalo semen freezability. Ejaculates (12) from 3 bulls (4 from each bull) were collected as per
standard procedure. After initial selection of ejaculates on the basis of mass activity ( + 3 and above) and individual
progressive motility (70% and above), ejaculates were divided into 3 parts and diluted with tris dilutor, G1, containing
5% EY powder; G2, containing 10% EY powder, and G3, containing fresh EY (20%). Ejaculates were processed
and cryopreserved using automated biological cell freezer followed by examination of frozen samples after 24 h.
Semen quality variables (individual progressive motility, viability using propidium iodide, acrosome integrity using
FITC-PSA and membrane integrity by HOST) were examined at fresh and post-thaw stage. At fresh stage semen
quality variables did not vary among 3 groups.
Not Available
 
Date 2021-10-05T09:10:19Z
2021-10-05T09:10:19Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64870
 
Language English
 
Relation Not Available;
 
Publisher Not Available