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Cryoinjury in relation to cholesterol content of bull spermatozoa

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Title Cryoinjury in relation to cholesterol content of bull spermatozoa
Not Available
 
Creator Srivastava N,
Srivastava S K
Ghosh S K,
Kumar A
Jerome A,
MAHAK SINGH
Kumar A
 
Subject Cholesterol,
Cryocapacitation
Cryoinjury
Pre-freeze
Spermatozoa
 
Description Not Available
Though semen cryopreservation has many advantages, it decreases tolerance of spermatozoa to stress of temperature variations,
thus disposing spermatozoa to increased cryoinjury. Predicting cryoinjury to spermatozoa is difficult with available laboratory
protocols. The present study was designed to evaluate the cryoinjury prediction of sperm in relation to its cholesterol
content. The cholesterol content at fresh, pre-freeze and post-thaw stage was 21.95±0.97, 9.58±0.45 and 7.44±0.44 ìg/100
million spermatozoa. Semen quality parameters (SQP) at pre-freeze and post-thaw stages were individual motility (71.58±1.29
vs. 47.83±1.16), acrosome integrity (71.00±1.48 vs. 61.58±1.70), Chlortetracycline (CTC) pattern (F: 29.67±1.14 vs.
15.83±1.02; B: 48.83±2.06 vs. 53.58±1.27; AR: 21.50±1.84 vs. 30.58±1.85). Study indicated that cholesterol content of
fresh spermatozoa could be used to predict cholesterol content of spermatozoa at pre-freeze and post-thaw stages with
medium to high level of accuracy. This study revealed strong evidence that cholesterol content of pre-freeze bull spermatozoa
could be a good predictor of level of cryoinjury following preservation at ultra low temperature.
Not Available
 
Date 2021-09-29T06:10:43Z
2021-09-29T06:10:43Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/64767
 
Language English
 
Relation Not Available;
 
Publisher Not Available