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Effect of reduced glutathione, water soluble vitamine E analogue and butylated hydroxytoluene on the post thaw charachterstics of boar spermatozoa.

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Title Effect of reduced glutathione, water soluble vitamine E analogue and butylated hydroxytoluene on the post thaw charachterstics of boar spermatozoa.
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Creator Baishya SK
Biswas RK
Kadirvel G
Deka BC
Sinha S
MAHAK SINGH
 
Subject Antioxidants
boar spermatozoa
cryopreservation
semen quality
 
Description Not Available
BACKGROUND: Antioxidant in freezing extender of boar semen improved post thaw sperm function. OBJECTIVE: The study compared the effects of reduced glutathione (GSH), water soluble vitamin E analogue Trolox and butylated hydroxytoluene (BHT) on quality of cryopreserved boar spermatozoa. MATERIALS AND METHODS: Using split sample technique three different antioxidants namely, GSH (1 mM), vitamin E (0.2 mM) and BHT (0.2 mM) were added to the freezing medium of lactose-egg yolk-glycerol extender, and samples were frozen using controlled freezing rate of 40C/min from -6 to -140C. Samples were evaluated for sperm motility, acrosomal status, plasma membrane integrity, mitochondrial membrane potential, lipid peroxidation and sperm DNA integrity after equilibration and after freezing. RESULTS: The supplementation of GSH, vitamin E and BHT resulted in significantly higher post thaw motility, live intact acrosome and plasma membrane intact sperm. The incidence of post thaw sperm lipid peroxidation was significantly reduced after addition of antioxidants. However, antioxidants treatment neither significantly improved mitochondrial membrane potential of live sperm sub-population nor sperm DNA integrity after freezing. There was no significant difference of the post thaw sperm characteristics among three antioxidants. Protective effect of GSH, vitamin E and BHT are comparable on cryopreserved boar spermatozoa.
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Date 2021-09-29T06:16:34Z
2021-09-29T06:16:34Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/64784
 
Language English
 
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Publisher Not Available