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Evaluation of designed consortium SNH-1 for efficient hydrolysis of agriculture waste to benefit bioethanol production

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Title Evaluation of designed consortium SNH-1 for efficient hydrolysis of agriculture waste to benefit bioethanol production
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Creator Shweta Srivastava
Nishant A. Dafale
Nilam Tulsani
Subhash J. Jakhasara
Niteen V. Patil
Chaitanya G. Joshi
Hemant J Purohit
 
Subject Microbial consortium
Agriculture waste hydrolysis
Optimization
Bioethanol
Genome sequencing
 
Description An environment friendly and cost-effective approach is a major bottleneck in biofuels production from agriculture waste residue. The present study unravels the enzymatic hydrolytic potential of a designed bacterial consortium (SNH-1) comprised of camel rumen derived B. safensis CRN 13, B. subtilis CRN 16, C. braakii CRN 21, B. circulans CRN 24, and P. dendritiformis CRN18. The consortium SNH-1 was evaluated for exo-glucanase (0.29 U/ml), β-glucosidase (0.69U/ml), β-glucuronidase (0.29U/ml), endoxylanase (0.79U/ml), arabinosidase (0.62U/ml), α-galactosidase (0.36 U/ml), and endoglucanase (0.51U/ml) activity. Genome analysis of consortium members identified 660 carbohydrate-active enzymes (CAZyme) responsible for breaking the structural intricacy. These include 296 glycoside hydrolase, 19 auxiliary activity, 186 glycosyl transferases, and 37 carbohydrate-binding modules genes. The designed consortium hydrolyzed sugarcane bagasse, wheat straw, and rice straw effectively. Response surface methodology (RSM) was applied to optimize the pH, temperature, and substrate concentration, which has resulted in a 23% higher saccharification of wheat straw. Further, 6.2% bioethanol production was estimated from wheat straw hydrolysate using S. cerevisiae. The wheat straw saccharification by the designed consortium is a cost-effective, chemical-free, cleaner, and promising approach for producing lignocellulosic bioethanol.
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Date 2022-03-16T10:01:00Z
2022-03-16T10:01:00Z
2021-03-15
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/70271
 
Language English
 
Relation Not Available;
 
Publisher Scientist Direct