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Actinomycetes isolates of arid zone of Indian Thar desert and efficacy of their bioactive compounds against human pathogenic bacteria

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Title Actinomycetes isolates of arid zone of Indian Thar desert and efficacy of their bioactive compounds against human pathogenic bacteria
Not Available
 
Creator Kumar, S.
Solanki., D. S.
Parihar, K.
Tak, A.
Gehlot, P.
Rakesh Pathak
Singh, S.K.
 
Subject 16S rRNA gene
Actinomycetes
Arid zone
Bioactive compounds
GC–MS
Indian Thar desert
 
Description Not Available
Twenty-six morphotypes of actinomycetes bacteria were isolated from the soils of arid zone of Indian Thar desert, Rajasthan. A significant and positive correlation was found between density of actinomycetes isolates and availability of nitrogen in sandy soil of arid zone suggesting the influence of soil nitrogen on occurrence and propagation of actinomycetes in this region. Molecular identification based on 16S rRNA gene sequencing revealed that the bacterial isolates belong to four actinomycetes genera, viz. Streptomyces (22 species), Nocardiopsis (two species), Saccharomonospora (one species) and Actinoalloteichus (one species). The preliminary screening of 26 isolates against five human pathogenic bacteria, viz. Escherichia coli, Vibrio cholera, Salmonella enterica typhimurium, Staphylococcus aureus and Enterococcus faecalis, showed that only four isolates, viz. Streptomyces sp. (ITD-27), S. enissocaesilis (ITD-29), S. Malachitospinus (ITD-35) and Streptomyces sp. (ITD-47), had antibacterial activity. The secondary screening of these four isolates revealed that the isolate S. malachitospinus (ITD-35) showed the maximum growth inhibition zone and inhibited the growth of all tested gram-positive and gram-negative pathogenic bacteria. Gas chromatography-mass spectrometry analysis of S. malachitospinus (ITD-35) cultural filtrate in n-butanol solvent identified three antibacterial compounds of medicinal significance, viz. 3-octanone, neopentyl isothiocyanate and 2-methyl butyl isothiocyanate.
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Date 2022-03-16T09:53:11Z
2022-03-16T09:53:11Z
2021-12-01
 
Type Research Paper
 
Identifier Kumar, S., Solanki., D. S., Parihar, K., Tak, A., Gehlot, P., Pathak. and Singh, S.K. 2021. Actinomycetes isolates of arid zone of Indian Thar desert and efficacy of their bioactive compounds against human pathogenic bacteria. Biologia Futura. Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/70269
 
Language English
 
Relation Not Available;
 
Publisher Biologia Futura