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Detection of endophytic association between Aeschynomene nodulating Bradyrhizobium sp. and traditional Desariya rice roots under rice‑Aeschynomene ecosystem of chaur land, Bihar, India

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Title Detection of endophytic association between Aeschynomene nodulating Bradyrhizobium sp. and traditional Desariya rice roots under rice‑Aeschynomene ecosystem of chaur land, Bihar, India
Not Available
 
Creator Abhilasha Rai
Devendra Singh
Manindra Nath Jha
Shobit Thapa
Sanjeet Kumar Chaurasia
Gopaljee Jha
 
Subject Aeschynomene
ARA assay
Artificial symbiosis
Desariya rice
Diazotrophic
 
Description Not Available
Engineering diazotrophic rice having either an integral component of diazotrophic microbes or placing microbial origin
nif gene to the rice plant is the dream of biotechnologist. Rice-Aeschynomene ecosystem of pristine chaur land provides a
suitable niche to search Rhizobium endophytes in rice. Accordingly, the work was initiated to search suitable endophytic
Rhizobium strain for artificial symbiosis within the roots of Desariya rice and its source through morphological, biochemical
and molecular approaches. Detection of Acetylene reduction assay (ARA) activity in sterilized Desariya rice root confirmed
the presence of putative diazotrophic endophytes in rice root. Isolates from Aeschynomene aspera L. nodulating and Desariya
rice endophytic Rhizobium were evaluated for growth, IAA, morphological and biochemical features. Carbon profiling pattern
of both these isolates indicated that Desariya rice endophytic Rhizobium has its similarity with Aeschynomene aspera
L. nodulating Rhizobium. 16S rRNA gene sequencing confirmed the presence of endophytic Bradyrhizobium sp. in Desariya
rice roots and its similarity with Aeschynomene aspera L. nodulating Bradyrhizobium. Desariya rice Bradyrhizobium may
be an ideal candidate in the future for creating artificial symbiosis in rice due to its similarity with Aeschynomene aspera
L. Bradyrhizobium.
Not Available
 
Date 2022-03-26T11:19:49Z
2022-03-26T11:19:49Z
2021-11-13
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/70710
 
Language English
 
Relation Not Available;
 
Publisher Not Available