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Optimization of tissue and time for rapid serological and molecular detection of apple stem pitting virus and Apple stem grooving virus in apple

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Title Optimization of tissue and time for rapid serological and molecular detection of apple stem pitting virus and Apple stem grooving virus in apple
Not Available
 
Creator S U NABI
J I MIR
O C SHARMA
D B SINGH
Z SHAFIA
M SHEIKH
M LUBNA
A K KAMRAN
 
Subject Apple . ASGV. ASPV.
Detection
Latent viruses
Real time PCR
 
Description Not Available
Majority of the apple trees are known to be
infected by two latent viruses, Apple stem grooving
virus (ASGV) and Apple stem pitting virus (ASPV).
The importance of ASGV and ASPV is due to their
non expression of symptoms, worldwide occurrence
and wide host range on pome and stone fruits. Due to
their latent nature in apple, early and rapid diagnostics
plays important role for production of virus free quality
planting material. The present investigation was conducted to detect and quantify ASPV & ASGV from
different plant parts (spatial) in apple trees during different seasons (temporal) for optimisation of tissue and
time for their rapid and early detection. Detection and
relative quantification using immuno-molecular diagnostic techniques like, Double Antibody SandwichELISA, Reverse Transcription-PCR and Real Time
RT-PCR in various plant parts (leaf, whole flower, sepal,
petal, anther, stigma with style, bark, fruit, seed and
root) during different seasons was done. The DASELISA based detection revealed infection in all plant
parts except root and fruit with ASGVand ASPV, showing more expression in leaves followed by bark and
whole flower. Similar results were also observed on
RT-PCR based detection. Quantitative real time PCR
analysis showed variation in expression of ASGV and
ASPVin different parts during different seasons. Results
confirmed that the ASGV and ASPV expression ishigher in leaves followed by bark and whole flower.
Periodic detection of these viruses in different plant
parts during all the four seasons revealed varied virus
titer from one season to another in the same plant.
During all the seasons, both ASPV and ASGV were
detected in bark in measurable titer using immunomolecular detection tools, however via DAS-ELISA,
ASGV remained undetected during dormant season.
Hence leaves and bark except leaf during fall, can be
directly used as detection material for their early and
rapid detection leading to production of virus free planting material.
Not Available
 
Date 2021-08-26T09:31:49Z
2021-08-26T09:31:49Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60806
 
Language English
 
Relation Not Available;
 
Publisher Springer