Isolation and identification of Bacillus amyloliquefaciens strains for bio-control of grapevine anthracnose
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Title |
Isolation and identification of Bacillus amyloliquefaciens strains for bio-control of grapevine anthracnose
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Creator |
Shubhangi Prakash Narkar, Indu Sanjay Sawant and Hemalata Govind Shete
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Subject |
Colletotrichum gloeosporioides, carbendazim, resistance, grape berry
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Description |
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In India, Colletotrichum gloeosporioides cause anthracnose disease of many horticultural crops, including grapes. Carbendazim resistance in the pathogen is commonly observed. Eighty-seven endophytic bacteria were isolated from mature shoots of Thompson Seedless variety located at ICAR-National Research Centre for Grapes, Pune and screened for their antagonistic activity. Ten bacteria inhibited the radial growth of cabendazim resistant C. gloeosporioides isolates by 77.59 - 84.59 per cent in dual culture assay. Four of these bacteria completely prevented anthracnose infections in detached leaf bio-assay and were subsequently evaluated at 10 to the power of 4, 10 to the power of 5, 10 to the power of 6 CFU/ml on field grown vines. Vines treated with bacteria TS-31, TS-45 and TS-46 recorded low disease severity even at the lowest concentration of 10 to the power of 4 CFU/ml and area under disease progress curve (AUDPC) were 456.31, 355.31 and 446.25, respectively; while in fungicide control and untreated control AUDPC was 435.19 and 756.88. In detached berries, TS-45 showed almost complete control of berry infection (2.384.12%), while TS-46 (20.393.33) and TS-31 (35.4632.65) were less effective. These three promising bio-control bacteria were identified as Bacillus anyloliquefaciens based on morphological, biochemical and molecular characterization. Not Available |
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Date |
2017-05-30T07:00:13Z
2017-05-30T07:00:13Z 2016-09-28 |
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Type |
Research Paper
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Identifier |
Shubhangi Prakash Narkar, Indu Sanjay Sawant and Hemalata Govind Shete. 2016. Isolation and identification of Bacillus amyloliquefaciens strains for bio-control of grapevine anthracnose. Journal of eco-friendly agriculture, 12: 62-66.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/4209 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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