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An improved pollen collection and cryopreservation method for highly recalcitrant tropical fruit species of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.)

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Title An improved pollen collection and cryopreservation method for highly recalcitrant tropical fruit species of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.)
Not Available
 
Creator Rekha Chaudhury, SK Malik, S Rajan
 
Subject CRYOPRESERVATION; CYCLOHEXANE; LITCHI; MANGO; POLLEN; VIABILITY
 
Description Not Available
An improved method for pollen collection from freshly dehiscing anthers of mango (Mangifera indica L.) and litchi (Litchi chinensis Sonn.) using the organic solvent cyclohexane has been devised. Using this method pollen quantity sufficient for large scale pollinations could be collected and stored for future use. Transport of pollen in viable conditions over long distances, from site of collection (field genebank) to cryolab was successfully devised for both these fruit species. Cryopreservation was successfully applied to achieve long-term pollen storage over periods of up to four years. Pollen viability was tested using in vitro germination, the fluorochromatic reaction (FCR) method and by fruit set following field pollination. On retesting, four year cryostored pollen of different mango and litchi varieties showed high percentage viability as good as fresh control pollens. Pollens of more than 180 cultivars of mango and 19 cultivars of litchi have been stored in the cryogenebank using the technology developed, thus facilitating breeding programmes over the long-term.
Not Available
 
Date 2017-10-17T07:22:21Z
2017-10-17T07:22:21Z
2010-05-01
 
Type Research Paper
 
Identifier 17
0143-2044 (Print); ISSN 1742-0644 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/5530
 
Language English
 
Relation Not Available;
 
Publisher Cryoletters