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Comparison of PCR based marker systems for genetic analysis in different cultivars of mango

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Title Comparison of PCR based marker systems for genetic analysis in different cultivars of mango
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Creator Navin Srivastava, Anju Bajpai, Ramesh Chandra, S Rajan, M Muthukumar, Manoj Kumar Srivastava
 
Subject Mango, Genetic relationship, Polymorphic information content, Marker index, Effective multiplex ratio, Diversity index
 
Description Not Available
Native diversity is well represented in northern and eastern parts of India for mango. We evaluated three
important polymerase chain reaction (PCR) based marker techniques viz., random amplified polymorphic
DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplified mini satellite DNA (DAMD) and
examined their suitability for depicting genetic relationships and discrimination among closely related group of 46
mango varieties grown in the different agro-ecological zones in Uttar Pradesh, Bihar and West Bengal. Nine
RAPD, eleven ISSR and four DAMD primers generated 110, 160 and 43 discrete fragments, respectively,
accounting for polymorphism of 87.3, 79.83 and 83.72%, respectively. Cumulative analysis of these markers
resulted in comprehensive UPGMA based dendrogram where in native mangoes representing important breeding
lines and varieties from Uttar Pradesh fall more or less in separate cluster, while Bihar and West Bengal cultivars
represent genetically different lineage forming distinct separate cluster. The prime focus on the study was towards
identification of genetic variability that warrants establishing origin and molecular evolution of mango cultivars of
eastern and northern India because they are the rich gene pool for conservation. Highest diversity index (DI) and
polymorphic information content (PIC) values were found in DAMD indicating it to be more informative than
others. Similarly, high effective multiplex ratio (EMR) and marker index (MI) were recorded by ISSR reflecting
ability to simultaneously detect a large number of bands. The study accomplished establishing genetic relationship and also DNA fingerprint development. The data is also useful for mapping studies for gene identification.
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Date 2017-10-17T08:03:37Z
2017-10-17T08:03:37Z
2012-01-01
 
Type Research Paper
 
Identifier 12
0254-8704
http://krishi.icar.gov.in/jspui/handle/123456789/5533
 
Language English
 
Relation Not Available;
 
Publisher Triveni Enterprises, Lucknow, India