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Yellows and corm rot in gladiolus: Incidence, identification and characterization of Fusarium oxysporum f. sp. gladioli

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Title Yellows and corm rot in gladiolus: Incidence, identification and characterization of Fusarium oxysporum f. sp. gladioli
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Creator Nitika Gupta, Prabha K., Ganesh B. Kadam, S. Sriram, N.K. Chandran
 
Subject Fusarium yellows, Fusarium oxysporum f. sp. gladioli, polymerase chain reaction,internal transcribed spacer region, translation elongation factor 1α
 
Description Not Available
To unravel the etiology of the plants showing yellowing, epinasty and wilting morphological and molecular
diagnostics was carried out. Colony morphology and microscopic examinations indicated the pathogen to be Fusarium
sp. To elucidate the identity conclusively, polymerase chain reaction (PCR) amplification of genomic region was done
using primers based on internal transcribed spacer (ITS) region and translation elongation factor (Tef) region. ITS
region of the pathogen showed 100 percent similarity to Fusarium oxysporum species and analysis of Tef region
helped to narrow down to Fusarium oxysporumf. sp. gladioli. Characterization by molecular techniques using PCR to
amplify the ITS and TEF 1α allows identifying organisms that cannot be distinguished morphologically and they can
also help to understand the mechanisms of pathogenic variation and therefore, to develop effective management
strategies.
Not Available
 
Date 2018-10-31T07:01:51Z
2018-10-31T07:01:51Z
2016-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/8807
 
Language English
 
Relation Not Available;
 
Publisher Not Available