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Serological detection of Grapevine leafroll-associated virus 4 in grapevine growing areas of India using polyclonal antiserum raised against the recombinant coat protein

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Title Serological detection of Grapevine leafroll-associated virus 4 in grapevine growing areas of India using polyclonal antiserum raised against the recombinant coat protein
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Creator Richa Raia, S.M.Paul Khurana, Sandeep Kumar, Nitika Gupta, V.K. Baranwala
 
Subject GLRaV-4 ACP-ELISA DIBA DAS-ELISA
 
Description Not Available
Grapevine leafroll disease (GLD), caused by a complex of serologically and genetically distinct or related viral
species termed as Grapevine leafroll associated viruses (GLRaVs), is a serious threat to the grape production
worldwide. Recently, Grapevine leafroll-associated virus 4 (GLRaV-4) has been reported in the Indian vineyards,
mostly as a mixed infection with GLRaV-3 and GLRaV-1. In order to screen the planting material, a polyclonal
antiserum against the in vitro expressed coat protein (CP) of GLRaV-4 was produced. The antiserum, purified
IgGs and conjugated IgGs detected GLRaV-4 in crude saps of infected grapevines and purified coat protein when
used in antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA), double antibody sandwich enzyme-
linked immunosorbent assay (DAS-ELISA), Western blotting and dot immunobinding assay (DIBA).
GLRaV-4 could be detected in 54% (39 out of 72) of symptomatic grapevine samples from different grape
growing regions of India using DAS-ELISA. The validation of DAS-ELISA results was done by RT-PCR using
GLRaV-4 CP-specific primers. To the best of our knowledge, this is the first report of a polyclonal antiserum
against the in vitro expressed CP of GLRaV-4 and its successful application in diagnosis of the virus in grape
vineyards of India
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Date 2018-11-01T04:22:57Z
2018-11-01T04:22:57Z
2018-03-30
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/8879
 
Language English
 
Relation Not Available;
 
Publisher Not Available