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Molecular characterization of banana bract mosaic virus from India reveals recombination and positive selection in the VPg gene

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Title Molecular characterization of banana bract mosaic virus from India reveals recombination and positive selection in the VPg gene
 
Creator Chelliah Anuradha & Ramasamy Selvarajan
 
Subject Banana bract mosaic virus . Viral genome-linked protein . Genetic diversity . Recombination . Selection pressure
 
Description Not Available
Banana bract mosaic virus (BBrMV), a member of the genus Potyvirus, family Potyviridae, is an important viral pathogen
affecting banana and plantains in India. The present study was undertaken to determine the genetic variation and molecular
evolution of BBrMV infecting different cultivars of banana and plantain in the Indian subcontinent, based on the viral genomelinked
protein (VPg) gene. Sequence identity of this gene from 29 BBrMV isolates showed a range of nucleotide (nt) and amino
acid (aa) identity of 75–100% and 95–100%, respectively. Phylogenetic analysis based on nt revealed that except for two isolates
(TN1 and TN2), all the other Indian isolates clustered together. Different functional motifs of the VPg gene previously reported
were found to be conserved. A single possible recombination event was detected using a recombination detection programme. A
codon-based selection analysis revealed that most of the codons in the VPg gene were under purifying selection except for those at
position 46, 47, 71, 107, 149, 153, 156, 175, 176, and 178, which were under positive selection. Gene flow between different
Indian populations of BBrMV from banana was relatively low. This is the first report on genetic diversity and evolution of the VPg
gene of BBrMV. Since VPg is identified as a virulence determinant in resistance mediated by the eukariotic intiation factor 4E
(eIF4E) in several plant-potyvirus interactions, any mutation in the VPg interacting domain may abolish the interaction with eIF4E
or its isoforms in vitro and prevent viral infection in planta. Therefore, understanding the population structure of BBrMV based on
VPg would potentially provide insight into the diversification and infection cycle of this virus.
Not Available
 
Date 2018-11-20T04:43:11Z
2018-11-20T04:43:11Z
1001-01-01
 
Type Article
 
Identifier https://doi.org/10.1007/s42161-018-0116-9.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/12184
 
Language English
 
Relation Not Available;
 
Publisher springer