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Rapid and sensitive detection of Pseudocercospora eumusae pathogen causing eumusae leaf spot disease of banana by loop-mediated isothermal amplification (LAMP) method

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Title Rapid and sensitive detection of Pseudocercospora eumusae pathogen causing eumusae leaf spot disease of banana by loop-mediated isothermal amplification (LAMP) method
 
Creator Thangavelu, R and Ganga Devi, P.
 
Subject Crude DNA Loop-Mediated Isothermal Amplification method Pseudocercospora eumusae Optimization
 
Description Not Available
A rapid and sensitive loop-mediated isothermal amplification (LAMP) method was developed for the specific detection of Pseudocercospora eumusae. The LAMP primers designed based on the specific SCAR (Sequence Characterized Amplified Region) marker sequence of P. eumusae proved highly specific to P. eumusae and there was no cross reactivity with closely related Pseudocercospora spp. (P. fijiensis and P. musicola) and 17 other leaf spot causing fungal pathogens of banana. The developed LAMP method exhibited greater sensitivity as the minimum detectable concentration of P. eumusae genomic DNA was 10 ƿg/µl which was 100 times lower than that of conventional PCR (1 ng/µl).This method also detected the target pathogen from crude DNA of the mycelium and single leaf spot tissues which eliminates laborious purification steps in DNA isolation and requires less operational time. To the best of our knowledge, this is the first report on the development of this LAMP method to specifically detect and diagnose P. eumusae pathogen from pure cultures and leaf spot tissues.
 
Date 2018-11-20T04:42:21Z
2018-11-20T04:42:21Z
2018-10-05
 
Type Research Paper
 
Identifier https://doi.org/10.1007/s13205-018-1468-8
2190-572X
http://krishi.icar.gov.in/jspui/handle/123456789/12182
 
Language English
 
Relation Not Available;
 
Publisher Springer International Publishing