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Genetic diversity among turf grasses by ISSR markers

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Title Genetic diversity among turf grasses by ISSR markers
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Creator A K TIWARI, G KUMAR, B TIWARI, G B KADAM and T N SAHA
 
Subject Genetic diversity, Geographical populations, Inter simple sequence repeat (ISSR), Turf grasses
 
Description Not Available
In this study, leaves sample of eight turf grass genera were used to standardized the ISSR protocol and quantities
of template DNA, dNTPs, MgCl2, Taq DNA polymerase, primer concentration and annealing temperature for each
primer. Analysis of molecular variance (AMOVA), Genetic diversity, Nei’s gene diversity, Shannon’s index, and Nei’s
unbiased genetic distance, partition, within- and among-group, of all parameters was analyzed. Levels of genetic
divergence between samples were calculated with the fixation index PhiPT. Statistics with AMOVA revealed 84 and
16 % variance among and within various mutants, respectively. Cluster analysis based on the Unweighted-pair group
method arithmetic average (UPGMA), Principal coordinate analysis (PCA) and Spatial correlation is a measured that
looks at the relationship (genetic distance) amongst mutants. PCOA analysis of ISSR data showed that the first three
factors comprised about 94.5% of total variance when the first, second and third axis comprised about 49.85, 29.29
and 15.36% of total variance, respectively. Statistically non-significant genetic similarity exists among population
which varies from -1.0000 to 0.2419. Maximum similarity was recorded between the two samples of Lolium perenne
and Agrostis stolonifera (0.0682) followed by Stenotaphrum secundatum, Poa pratensis (0.0419). Cluster analysis
was conducted to generate a dendogram elucidating for relationships among turf grass. The first cluster divided into
three sub-clusters comprising: (i) Poa pratensis and Agrostis stolonifera (ii) DFR-NS-1 and Lolium perenne and (iii)
the DFR-NS-1 Festuca arundinacea.The second cluster consisted of Stenotaphrum secundatum, Zyosia junsia and
Paspalum vaginatum. Within the second cluster Paspalum vaginatum was separated from the zoysiagrass. As revealed
by ISSR analysis, Stenotaphrum secundatum, and Paspalm were quite distinct from bermudagrass (DFR-NS-1) and
rest of the turf grasses.
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Date 2018-11-14T10:37:40Z
2018-11-14T10:37:40Z
2017-02-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/Publication/handle/123456789/11245
 
Language English
 
Relation Not Available;
 
Publisher Not Available