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Development, identification and validation of CAPS marker for SHELL trait which governs dura, pisifera and tenera fruit forms in oil palm (Elaeis guineensis Jacq.)

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Title Development, identification and validation of CAPS marker for SHELL trait which governs dura, pisifera and tenera fruit forms in oil palm (Elaeis guineensis Jacq.)
Not Available
 
Creator B. Kalyana Babu
R. K. Mathur
P. Naveen Kumar
D. Ramajayam
G. Ravichandran
M. V. B. Venu
 
Subject SHELL, dura, pisifera and tenera, CAPS marker
 
Description Not Available
The oil palm fruit forms (dura, pisifera and tenera) governed by the shell thickness gene (Sh)
plays a major role in identification of fruit type and also influences palm oil yield. Identification
of desired fruit type is a major asset to the breeders and oil palm workers for applications in
breeding, seed certification and to reduce time, space and money spent on identification of
fruit form. In the present study, we developed Sh gene specific primer pairs and bulk segregant
analysis was done using 300 genomic and 8 genic SSR markers. We identified one
cleaved amplified polymorphic site (CAPS) marker for differentiation of oil palm fruit type
which produced two alleles (280 and 250bp) in dura genotypes, three alleles in tenera genotypes
(550, 280, and 250bp) and one allele in pisifera genotypes (550bp). The shell allele
sequencing results showed that two SNPs were present, of which SNP2 contributed for variation
of fruit forms. The nucleotide `A' was present in only dura genotypes, where as `T' was
present only in pisifera genotypes, which in turn led to the change of amino acid lysine to
aspargine. The identified CAPS marker was validated on 300 dura, 25 pisifera and 80 tenera
genotypes, 80 dura/ pisifera cross progenies and 60 lines of tenera/ tenera cross progeny.
Association mapping of marker data with phenotypic data of eight oil yield related traits
resulted in identification of seven significant QTLs by GLM approach, four by MLM approach
at a significant threshold (P) level of 0.001. Significant QTLs were identified for fruit to bunch
and oil to bunch traits, which explained R2 of 12.9% and 11.5% respectively. The CAPS
marker used in the present study facilitate selection and timely distribution of desirable high
yielding tenera sprouts to the farmers instead of waiting for 4±5 years. This saves a lot of
land, time and money which will be a major breakthrough to the oil palm community.
Not Available
 
Date 2018-11-19T07:49:24Z
2018-11-19T07:49:24Z
2017-02-01
 
Type Article
 
Identifier Babu BK, Mathur RK, Kumar PN, Ramajayam D, Ravichandran G, Venu MVB, et al. (2017) Development, identification and validation of CAPS marker for SHELL trait which governs dura, pisifera and tenera fruit forms in oil palm (Elaeis guineensis Jacq.). PLoS ONE 12(2): e0171933. doi:10.1371/journal.pone.0171933
doi:10.1371/journal.pone.0171933
http://krishi.icar.gov.in/jspui/handle/123456789/12007
 
Language English
 
Relation Not Available;
 
Publisher PLOS one