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Mapping QTLs for opaque2 modifiers influencing the tryptophan content in quality protein maize using genomic and candidate gene-based SSRs of lysine and tryptophan metabolic pathway

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Title Mapping QTLs for opaque2 modifiers influencing the tryptophan content in quality protein maize using genomic and candidate gene-based SSRs of lysine and tryptophan metabolic pathway
 
Creator B. Kalyana Babu
P. K. Agrawal
S. Saha
H. S. Gupta
 
Subject Maize Opaque2 modifiers Tryptophan QTL mapping Candidate genes QTL cartographer
 
Description Not Available
Key message The mapping analysis resulted in identification
of five significant QTLs for opaque2 modifiers
influencing the tryptophan content in quality protein
maize using functional and genomic SSR markers.
Abstract Quality protein maize (QPM) was developed by
selecting genetic modifiers that convert opaque2 mutant
containing high lysine and tryptophan. There are several
unlinked opaque2 modifier loci (Opm) in QPM whose
location, nature and mode of action are not clear. To
identify these Opm QTLs, we developed a population of
218 F2:3 individuals from a cross between VQL2 and
VQL8, two isogenic QPM inbreds significantly differing in
tryptophan content. Based on the data of the F2:3 population,
five significant QTLs on chromosomes 5, 7 and 9 with
LOD values more than 2.5 were identified and together
explained 38.6 % of the total phenotypic variance (R2).
The Wx1 gene which has influence on the amino acid
composition of the maize endosperm was mapped on
chromosome 9 near the marker phi022 and also validated
by bulk analysis. The QTL near the SSR marker ZmASK3,developed from the aspartate kinase 2 gene of the lysine
pathway, mapped on chromosome 5 and had LOD of 2.7
with R2 of 5.1 %. On chromosome 9, the QTL between the
loci umc1430 and bnlg1401 had an LOD of 4.5 with R2 of
9.1 %, whereas the QTL between the loci bnlg1401 and
phi022 had an LOD of 4.2 with R2 of 8.4 %. The third QTL
was observed to be close to the marker umc2207 with an
LOD of 4.8 and R2 of 8.4 %. The identified QTLs will be
very useful in the marker-assisted back-cross breeding and
transgressive breeding for the development of QPM maize.
Not Available
 
Date 2019-12-07T09:21:41Z
2019-12-07T09:21:41Z
2015
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28648
 
Language English
 
Relation Not Available;
 
Publisher Springer