Suppression of growth and endopeptidases of red palm weevil, Rhynchophorus ferrugineus (Olivier), infesting coconut using proteinase inhibitors.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Suppression of growth and endopeptidases of red palm weevil, Rhynchophorus ferrugineus (Olivier), infesting coconut using proteinase inhibitors.
Not Available |
|
Creator |
Not Available
Josephrajkumar, A., Chandrika Mohan and Chaturvedi, V.K. |
|
Subject |
Rhynchophorus ferrugineus, gut proteinase, proteinase inhibitors, aprotinin, soybean trypsin inhibitor
|
|
Description |
Not Available
Investigations on luminal proteinases of grubs of red palm weevil, Rhynchophorus ferrugineus (Olivier) [Curculionidae: Coleoptera] infesting coconut revealed the presence of two endodpeptidases viz., trypsin (BApNA-ase activity) and elastase-like chymotrypsin (SAAPLpNA-ase activity) in all stages of larval development. Highest activity of these proteinases coincided with the active feeding stage (mid-larval stage) of the insect. Results indicated that 50 g of aprotinin, 50 g of Soybean Trypsin Inhibitor (SBTI) and 1700 g of Phenyl Methyl Sulphonyl Fluoride (PMSF) inhibited trypsin activity of R. ferrugineus by 77.4%, 63.1% and 55.9%, respectively. Serine proteinase inhibitors viz., aprotinin (50 g), SBTI (50 g) and PMSF (1700 g) had a marginal reduction of elastase-like chymotrypsin activity of R. ferrugineus by 32%, 14% and 11%, respectively suggesting the serine nature of the proteinase. Aprotinin caused higher inhibition than SBTI and PMSF for both the proteinases studied. In vivo bioassay of 250 M aprotinin on coconut petiole method using early stage grubs of R. ferrugineus indicated a significant weight loss of 18.9% due to incorporation of serine proteinase inhibitor, aprotinin in a period of 120 h. Possibility of using serine proteinase inhibitor, aprotinin in the management of R. ferrugineus was suggested. Not Available Not Available |
|
Date |
2018-11-26T04:18:22Z
2018-11-26T04:18:22Z 2017-02-18 |
|
Type |
Research Paper
|
|
Identifier |
Entomon
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/13237 |
|
Language |
English
|
|
Relation |
Not Available;
41(4);283-293 |
|
Publisher |
Association for Advancement of Entomology
|
|