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Suppression of growth and endopeptidases of red palm weevil, Rhynchophorus ferrugineus (Olivier), infesting coconut using proteinase inhibitors.

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Title Suppression of growth and endopeptidases of red palm weevil, Rhynchophorus ferrugineus (Olivier), infesting coconut using proteinase inhibitors.
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Creator Not Available
Josephrajkumar, A., Chandrika Mohan and Chaturvedi, V.K.
 
Subject Rhynchophorus ferrugineus, gut proteinase, proteinase inhibitors, aprotinin, soybean trypsin inhibitor
 
Description Not Available
Investigations on luminal proteinases of grubs of red palm weevil, Rhynchophorus ferrugineus (Olivier) [Curculionidae: Coleoptera] infesting coconut revealed the presence of two endodpeptidases viz., trypsin (BApNA-ase activity) and elastase-like chymotrypsin (SAAPLpNA-ase activity) in all stages of larval development. Highest activity of these proteinases coincided with the active feeding stage (mid-larval stage) of the insect. Results indicated that 50 g of aprotinin, 50 g of Soybean Trypsin Inhibitor (SBTI) and 1700 g of Phenyl Methyl Sulphonyl Fluoride (PMSF) inhibited trypsin activity of R. ferrugineus by 77.4%, 63.1% and 55.9%, respectively. Serine proteinase inhibitors viz., aprotinin (50 g), SBTI (50 g) and PMSF (1700 g) had a marginal reduction of elastase-like chymotrypsin activity of R. ferrugineus by 32%, 14% and 11%, respectively suggesting the serine nature of the proteinase. Aprotinin caused higher inhibition than SBTI and PMSF for both the proteinases studied. In vivo bioassay of 250 M aprotinin on coconut petiole method using early stage grubs of R. ferrugineus indicated a significant weight loss of 18.9% due to incorporation of serine proteinase inhibitor, aprotinin in a period of 120 h. Possibility of using serine proteinase inhibitor, aprotinin in the management of R. ferrugineus was suggested. Not Available
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Date 2018-11-26T04:18:22Z
2018-11-26T04:18:22Z
2017-02-18
 
Type Research Paper
 
Identifier Entomon
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13237
 
Language English
 
Relation Not Available;
41(4);283-293
 
Publisher Association for Advancement of Entomology