Microsatellites markers to study genetic relationships among cowpea (Vigna unguiculata) genotypes
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Title |
Microsatellites markers to study genetic relationships among cowpea (Vigna unguiculata) genotypes
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Creator |
HIRA LAL, NAGENDRA RAI, KRISHNA KUMAR RAI and SHAILESH KUMAR TIWARI
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Subject |
Cowpea, Genetic diversity, Germplasm management, Microsatellites, Vigna unguiculata
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Description |
Not Available
Genetic diversity and phylogenetic relationships among 48 cowpea [Vigna unguiculata (L.) Walp.] genotypes collected from different places in India were evaluated using simple sequence repeat molecular markers. A set of 40 primer combinations developed from cowpea enomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea genotypes. Eleven primer combinations detected polymorphisms; sixteen primer combinations were monomorphic, with the remaining 13 primer sets failing to yield PCR amplification products. From one to 5 alleles were found among the informative primer combinations. The genetic diversity of the sample varied from 0.02 to 0.39 (mean = 0.205). The polymorphic information content ranged from 0.12 to 0.86 (mean = 0.49). The inter-cluster representatives of distant clusters (based on morphological and molecular markers) would be more useful for choosing the parents in cowpea breeding programmes. Not Available |
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Date |
2018-11-24T08:27:53Z
2018-11-24T08:27:53Z 2016-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/13108 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Journal of Agricultural Sciences
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