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Microsatellites markers to study genetic relationships among cowpea (Vigna unguiculata) genotypes

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Title Microsatellites markers to study genetic relationships among cowpea (Vigna unguiculata) genotypes
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Creator HIRA LAL, NAGENDRA RAI, KRISHNA KUMAR RAI and SHAILESH KUMAR TIWARI
 
Subject Cowpea, Genetic diversity, Germplasm management, Microsatellites, Vigna unguiculata
 
Description Not Available
Genetic diversity and phylogenetic relationships among 48 cowpea [Vigna unguiculata (L.) Walp.] genotypes collected from different places in India were evaluated using simple sequence repeat molecular markers. A set of 40 primer combinations developed from cowpea enomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea genotypes. Eleven primer combinations detected polymorphisms; sixteen primer combinations were monomorphic, with the remaining 13 primer sets failing to yield PCR amplification
products. From one to 5 alleles were found among the informative primer combinations. The genetic diversity of the
sample varied from 0.02 to 0.39 (mean = 0.205). The polymorphic information content ranged from 0.12 to 0.86
(mean = 0.49). The inter-cluster representatives of distant clusters (based on morphological and molecular markers)
would be more useful for choosing the parents in cowpea breeding programmes.
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Date 2018-11-24T08:27:53Z
2018-11-24T08:27:53Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/13108
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Agricultural Sciences