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Screening of Tomato Genotypes Against Root-Knot Nematode and Validation of Mi 1 Gene Linked Markers

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Title Screening of Tomato Genotypes Against Root-Knot Nematode and Validation of Mi 1 Gene Linked Markers
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Creator Yerasu Suresh Reddy• C. Sellaperumal • H. C. Prasanna• Akhilesh Yadav Sarvesh P. Kashyap • Satyedra Singh • Nagender Rai • Major Singh B. Singh
 
Subject Root-knot nematode , Mi 1 gene , Marker assisted selection , Co-dominant markers
 
Description Not Available
In India, tomato (Solanum lycopersicum L.) suffers yield loss between 11 and 35 % due to root-knot nematode (RKN) (Meloidogyne spp.) infestation. Mi 1 gene in tomato confers resistance to the three most damaging RKN species viz, Meloidogyne incognita, M. arenaria and M. javanica. The loss can be averted through the use of cultivars containing Mi 1 gene or incorporating the gene in new cultivars. To identify resistant genotypes, in 2013–2014 a total of 32 genotypes were screened against M. incognita at 2000 J2/Kg inoculum. Genotypes, Motelle and H-88-78-1 have showed immune reaction. Mogor and Hisar Lalit also showed resistance reaction. In 2014–2015, nine genotypes including four immune/resistant lines from the last year were screened against RKN at two inoculation rates of 2000 and 4000 J2. Reaction of Motelle was similar at both inoculum levels. In other resistant genotypes there was increase in mean of gall index when the inoculum levels were increased. The genetic background had an effect on the variations observed in number of galls produced in the resistant genotypes. Marker assisted selection for resistant genes greatly enhance selection of resistant plants in breeding. To identify suitable marker, six polymerase chain reaction based co-dominant markers were used for molecular screening of Mi 1 gene. Four markers namely, REX-1, CT119, Mi23 and Pmi gave banding pattern in accordance with the reported literature. Considering tight linkage with the gene and assay cost twosequences characterized amplified region markers, Mi23 and Pmi can be preferred over other markers for selecting Mi 1 containing genotypes.
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Date 2018-11-24T08:27:46Z
2018-11-24T08:27:46Z
2016-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/13107
 
Language English
 
Relation Not Available;
 
Publisher Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. , Springer