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Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut

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Title Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut
Not Available
 
Creator Kaitheri Edathil Rachana
Sudalaimuthu Asari Naganeeswaran
Thayale Purayil Fayas
Regi Jacob Thomas
Muliyar Krishna Rajesh
 
Subject Coconut
homology modeling
nucleotide binding site-leucine rich repeat type
real time PCR
resistant gene analogue
 
Description Not Available
Coconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant
cultivar Chowghat Green Dwarf (CGD) by using primers designed based on conserved motifs of the
NBS-LRR domain of the date palm. The amplifi ed sequences were cloned, sequenced and characterized. The
coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of
the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction
analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease
resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease
resistant traits in coconut.
Not Available
 
Date 2018-11-26T11:24:43Z
2018-11-26T11:24:43Z
2016-01-01
 
Type Article
 
Identifier Acta Bot. Croat. 75 (1), 1–10, 2016
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13471
 
Language English
 
Relation Not Available;
 
Publisher Not Available