Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut
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Title |
Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut
Not Available |
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Creator |
Kaitheri Edathil Rachana
Sudalaimuthu Asari Naganeeswaran Thayale Purayil Fayas Regi Jacob Thomas Muliyar Krishna Rajesh |
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Subject |
Coconut
homology modeling nucleotide binding site-leucine rich repeat type real time PCR resistant gene analogue |
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Description |
Not Available
Coconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant cultivar Chowghat Green Dwarf (CGD) by using primers designed based on conserved motifs of the NBS-LRR domain of the date palm. The amplifi ed sequences were cloned, sequenced and characterized. The coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease resistant traits in coconut. Not Available |
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Date |
2018-11-26T11:24:43Z
2018-11-26T11:24:43Z 2016-01-01 |
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Type |
Article
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Identifier |
Acta Bot. Croat. 75 (1), 1–10, 2016
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/13471 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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