Record Details

Cryopreservation of arecanut (Areca catechu L.) pollen

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Cryopreservation of arecanut (Areca catechu L.) pollen
Not Available
 
Creator Anitha Karun
Sajini, K.K.
Muralikrishna, K.S.
Rajesh, M.K.
Florent Engelmann
 
Subject arecanut
pollen
cryopreservation
germination
vigour
fertility
 
Description Not Available
BACKGROUND: Cryopreservation opens new avenues in the field of genetic resource conservation, especially in recalcitrant seeded palms such as arecanut for which field genebanks are exposed to pest and disease attacks and natural calamities, It is only through cryopreservation that the safety of the conserved germplasm can be assured at a relatively low cost for extended periods, OBJECTIVE: The objective of this work was to standardize various aspects of arecanut pollen cryopreservation, viz, collection and desiccation of pollen, in vitro germination, viability and fecundity studies, MATERIALS AND METHODS: Pollens of three arecanut genotypes (Sumangala, Hirehalli Dwarf and Hirehalli Dwarf x Sumangala) were collected in December 2013-February 2014, In vitro viability tests were conducted using fresh and desiccated pollen, Desiccated pollen was cryopreserved by direct immersion in liquid nitrogen and cryostored for different durations (24 hours to 2 years),
Viability and fertility studies were conducted using cryopreserved pollen, RESULTS: Pollen extraction
was achieved from fully opened male flowers by desiccation at room temperature (33-34°C), A medium containing 2,5 giL sucrose was found to be best for in vitro germination at room temperature. There was no significant difference in germination between desiccated and cryopreserved pollen whereas pollen tube length decreased significantly after cryopreservation, Fertility studies using HD x Sumangala pollen cryostored for various durations (l month, 1 year and 2 years) showed the setting of 70, 43 and 62%, respectively, Normal nut set was observed using cryopreserved pollen. CONCLUSION: Pollen cryopreservation is a viable option for germplasm conservation and hybridization programmes in arecanut.
Not Available
 
Date 2018-11-26T11:43:43Z
2018-11-26T11:43:43Z
1001-01-01
 
Type Article
 
Identifier CryoLetters 38(6),463-470(2017)
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13477
 
Language English
 
Relation Not Available;
 
Publisher Not Available