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Characterization of Three Different Chitinase Genes from Trichoderma asperellum Strain Ta13

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Title Characterization of Three Different Chitinase Genes from Trichoderma asperellum Strain Ta13
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Creator R. Thava Prakasa Pandian, M. Raja and Pratibha Sharma
 
Subject Cell wall degrading enzymes Chitinase genes Phylogenetic analysis Trichoderma asperellum
 
Description Not Available
Trichoderma asperellum is well-known for the
biocontrol activity against diverse plant pathogens. Various
cell wall degrading enzymes viz. chitinases, glucanases and
proteases are produced by T. asperellum against plant
pathogens. Chitinases are the main class of enzyme that
operate against fungal pathogen cell wall. In the present
study, a total of three different chitinase genes viz. Chit42,
Chit2 and ChitB were cloned and characterized from T.
asperellum strain Ta13. The phylogenetic analysis of three
different chitinase gene sequences formed separate cluster
among themselves. Sequence similarity analysis of the
nucleotide and amino acid sequences showed the uniqueness
and distinct nature of the amplified chitinase genes.
Maximum chitinase activity (93.5 mg/ml NAGA concentration)
revealed that Ta13 is an efficient chitinase producer
which acts against agriculturally important pathogens.
Not Available
 
Date 2018-12-01T11:03:21Z
2018-12-01T11:03:21Z
2017-09-21
 
Type Article
 
Identifier Not Available
0369-8211
http://krishi.icar.gov.in/jspui/handle/123456789/14933
 
Language English
 
Relation Not Available;
 
Publisher The National Academy of Sciences, India (Springer)