Characterization of Three Different Chitinase Genes from Trichoderma asperellum Strain Ta13
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Title |
Characterization of Three Different Chitinase Genes from Trichoderma asperellum Strain Ta13
Not Available |
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Creator |
R. Thava Prakasa Pandian, M. Raja and Pratibha Sharma
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Subject |
Cell wall degrading enzymes Chitinase genes Phylogenetic analysis Trichoderma asperellum
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Description |
Not Available
Trichoderma asperellum is well-known for the biocontrol activity against diverse plant pathogens. Various cell wall degrading enzymes viz. chitinases, glucanases and proteases are produced by T. asperellum against plant pathogens. Chitinases are the main class of enzyme that operate against fungal pathogen cell wall. In the present study, a total of three different chitinase genes viz. Chit42, Chit2 and ChitB were cloned and characterized from T. asperellum strain Ta13. The phylogenetic analysis of three different chitinase gene sequences formed separate cluster among themselves. Sequence similarity analysis of the nucleotide and amino acid sequences showed the uniqueness and distinct nature of the amplified chitinase genes. Maximum chitinase activity (93.5 mg/ml NAGA concentration) revealed that Ta13 is an efficient chitinase producer which acts against agriculturally important pathogens. Not Available |
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Date |
2018-12-01T11:03:21Z
2018-12-01T11:03:21Z 2017-09-21 |
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Type |
Article
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Identifier |
Not Available
0369-8211 http://krishi.icar.gov.in/jspui/handle/123456789/14933 |
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Language |
English
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Relation |
Not Available;
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Publisher |
The National Academy of Sciences, India (Springer)
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