Record Details

Comparative characterization of small RNAs derived from an emaravirus and a geminivirus infecting pigeonpea

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Comparative characterization of small RNAs derived from an emaravirus and a geminivirus infecting pigeonpea
 
Creator Basavaprabhu L. Patil*
Deepika Arora
 
Subject Emaravirus
small RNA
Geminivirus
 
Description Not Available
High throughput sequencing technologies, supported by bioinformatics tools are employed to retrieve small RNA sequence information derived from the nucleic acids of plant infecting viruses. In addition to characterization of the small RNAs to understand the biology of the virus, the small RNA sequence can be assembled to reconstitute viral genome sequence. For the first time the semiconductor based Ion Proton sequencing technology is used to sequence the small RNAs from pigeonpea (Cajanus cajan) plants infected by two distinct viruses with RNA and DNA as their genomes. The reconstitution of the viral genome sequence revealed that the pigeonpea plant from Kalaburagi (erstwhile Gulbarga, Karnataka state) was infected by an emaravirus species Pigeonpea sterility mosaic emaravirus 1 (PPSMV-1) and another plant from New Delhi
was infected by a begomovirus species Mungbean yellow mosaic India virus (MYMIV). Characterization and comparison of small RNA sequences derived from both the viruses showed vast differences in their pattern of accumulation and their size classes. In the case of PPSMV-1, the 21 nt sized siRNAs accumulated at far greater levels followed by 22 and 24 nt siRNAs. Whereas in MYMIV, the proportion of accumulation of each size class of siRNAs was similar. Further the distribution of small RNAs across the genomes of PPSMV-1 and MYMIV was mapped and the density of small RNA accumulation showed a positive correlation with the GC content of viral sequence.
Not Available
 
Date 2019-03-18T08:16:51Z
2019-03-18T08:16:51Z
2018-10-01
 
Type Journal
 
Identifier B. L. Patil* and D. Arora (2018) Comparative characterization of small RNAs derived from an emaravirus and a geminivirus infecting pigeonpea. Journal of Plant Biochemistry and Biotechnology 27(4):382–392.
https://doi.org/10.1007/s13562-018-0447-9(0123456789().,-volV)(0123456789().,-volV)
http://krishi.icar.gov.in/jspui/handle/123456789/17369
 
Language English
 
Relation Not Available;
 
Publisher Journal of Plant Biochemistry and Biotechnology