Record Details

Variability of emaravirus species associated with sterility mosaic disease of pigeonpea in India provides evidence of segment reassortment

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Variability of emaravirus species associated with sterility mosaic disease of pigeonpea in India provides evidence of segment reassortment
Not Available
 
Creator Basavaprabhu L. Patil*
Meenakshi Dangwal
Ritesh Mishra
 
Subject Emaravirus
 
Description Not Available
Sterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), with genomes consisting of five and six negative-sense RNA segments, respectively. The recently published genome sequences of both PPSMV-1 and PPSMV-2 are from a single location, Patancheru from the state of Telangana in India. However, here we present the first report of sequence variability among 23 isolates of PPSMV-1 and PPSMV-2, collected from ten locations representing six states of India. Both PPSMV-1 and PPSMV-2 are shown to be present across India and to exhibit considerable sequence variability. Variability of RNA3 sequences was higher than the RNA4 sequences for both PPSMV-1 and PPSMV-2. Additionally, the sixth RNA segment (RNA6), previously reported to be associated with only PPSMV-2, is also associated with isolates of PPSMV-1. Multiplex reverse transcription PCR (RT-PCR) analyses show that PPSMV-1 and PPSMV-2 frequently
occur as mixed infections. Further sequence analyses indicated the presence of reassortment of RNA4
between isolates of PPSMV-1 and PPSMV-2.
Not Available
 
Date 2019-03-18T08:17:21Z
2019-03-18T08:17:21Z
2017-07-11
 
Type Journal
 
Identifier B. L. Patil*, M. Dangwal and R. Mishra. (2017) Variability of emaravirus species associated with sterility mosaic disease of pigeonpea in India provides evidence of segment reassortment. Viruses. 9(7). pii: E183.
doi:10.3390/v9070183
http://krishi.icar.gov.in/jspui/handle/123456789/17370
 
Language English
 
Relation Not Available;
 
Publisher Viruses