Record Details

Cloning and characterization of protease inhibitor genes from some legumes

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Cloning and characterization of protease inhibitor genes from some legumes
Not Available
 
Creator Manoj Saini, P. Nandeesha, Mayank Kaashyap, Prasoonpal Gupta, Mukesh Mohan and Subhojit Datta
 
Subject Proteinase inhibitor, Insect resistance, Legumes
 
Description Not Available
Protease inhibitors are natural defense proteins that inhibit the proteolytic activity of proteases. Legume protease inhibitors
are diverse in their molecular mechanism and many of them have successfully been deployed in crop plants to confer
resistance against insect pests. Nine combinations of degenerated primers,designed, based on “multiple aligned” gene
sequences from NCBI Gene bank database were used to amplify the protease inhibitor genes in of ten different legume
crops (two varieties each)viz.fieldpea,chickpea, lentil, lathyrus, pigeonpea , frenchbean,mungbean ,clusterbean ,
horsegramand cowpea .The primer combination BBF2/BBR2 amplified the expectedamplicon of 800 bp invarities
ofmungbean,horsegramand cowpea. These amplicons obtained from each genotype were further ligated to the pJET1.2/
blunt vector and transformed into XL1 blue strain of E.coli. Out of 41 clones sequenced, two clone sequences obtained
from cowpea (DCS 6) showed homology with substilisin-like serine protease inhibitor of Desulfovibria vulgaris and
serine/threonine protein kinase of Epichlocfastucae.These genes can be transformed into Agrobacterium based binary
vectors and used for transformation of crops. Therefore these findings offer new genes for transgenic development against
lepidopteron pests.
Not Available
 
Date 2019-03-18T08:19:35Z
2019-03-18T08:19:35Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17377
 
Language English
 
Relation 38 (2): 178-181e;
 
Publisher Legume Research