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Transformation of Tomato with cry2AX1 gene of Bacillus thuringiensis

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Title Transformation of Tomato with cry2AX1 gene of Bacillus thuringiensis
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Creator Ruturaj Barthake R., A. Naveenkumar, P. Nandeesha, R. Manikandan, N. Balakrishnan, V. Balasubramani, D. Sudhakar, and V. Udayasuriyan
 
Subject cry2AX1, regeneration, Helicoverpa armigera, tomato transformation
 
Description Not Available
Tomato (Solanum lycopersicum L.,) is an important vegetable crop extensively damaged by lepidopteran insect
pest, Helicoverpa armigera Hubner (tomato fruit borer). In order to mitigate this problem, an attempt was made to generate
transgenic tomato plants resistant to fruit borer. In this study, a regeneration protocol was standardized for a local high yielding
genotype, cv. PKM1. Out of nine different combinations of media tested, modified MS medium containing B5 vitamins along
with zeatin exhibited higher regeneration efficiency. Cotyledonary explants of cv. PKM1 were transformed with an indigenous
synthetic cry2AX1 gene encoding insecticidal crystal protein of Bacillus thuringiensis through Agrobacterium mediated
transformation. Screening by PCR revealed presence of cry2AX1 gene in five out of nine putative transformants; two of the five
PCR positive transformants showed expression of Cry2AX1 protein in qualitative ELISA. The two ELISA positive plants
showed low level of mortality in H. armigera and significant reduction in feeding of leaf area, in detached leaf bioassay. Selection
of cry2AX1 transgenic tomato plants by quantitative ELISA for high level of gene expression will be useful for getting plants
with desirable level of insect protection.
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Date 2019-03-18T08:20:27Z
2019-03-18T08:20:27Z
2014-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17380
 
Language English
 
Relation Not Available;
 
Publisher SERIALS PUBLICATIONS