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An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis

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Title An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis
Not Available
 
Creator Alok Das & Sumit Kumar & P. Nandeesha & Indu Singh Yadav & Jyoti Saini & S. K. Chaturvedi & Subhojit Datta
 
Subject Organogenesis . Regeneration . Auxins . Cytokinins . Micro-grafting
 
Description Not Available
In-vitro regeneration in fieldpea was achieved
from immature embryonic axes and cotyledonary node
explants of six genotypes on modified MS media
supplemented with different concentration of plant growth
regulators, 6-Benzylamino purine (BAP) and Naphthalene
acetic acid (NAA). The best regeneration response, leading
to multiple shoot formation efficiency (22.34 shoots/explant)
was observed in the medium supplemented with 1.0 mg/L
BAP and 0.2 mg/L NAA and best frequency (67.55±4.74)
was achieved on medium containing 2.0 mg/L BAP and
0.4 mg/L NAA. The shoots were subcultured on a medium
supplemented with a combination of 1.0 mg/L GA3, 2.0 mg/L
BAP and 0.4 mg/L NAA, which resulted in elongation of
85 % of shoots. Rooting attempted from the elongated shoots,
on half strength MS medium and supplemented with three
different auxins IBA, IAA and NAA separately, exhibited
similar results. Alternatively, micro-grafting of in vitro
regenerated shoots onto pre-germinated root stocks raised in
green house facility was attempted with high success rate
(75 %). The grafted plants could be successfully hardened,
fertigated with Hoagland solution and distilled water in
a ratio of (1:10) for acclimatization and further development.
All the genotypes tested, produced multiple shoots that
could be established to mature fertile plant, hence, the
medium combinations used were found to be genotype
neutral.
Not Available
 
Date 2019-03-18T08:21:13Z
2019-03-18T08:21:13Z
2014-04-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17383
 
Language English
 
Relation Not Available;
 
Publisher springer