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Phylogenetic Analysis of Pulse Crops Using RAPD and Seed Storage Protein Markers

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Title Phylogenetic Analysis of Pulse Crops Using RAPD and Seed Storage Protein Markers
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Creator RINKI DEVIa, NANDEESHA Pa, MAYANK KAASHYAPa, SHIV KUMARa,b and SUBHOJIT DATTA*a
 
Subject Genetic diversity, Pulse crops, RAPD, SDS-PAGE, storage protein
 
Description Not Available
RAPD and SDS-PAGE techniques were employed for identification and genetic diversity estimation of four major pulses,
namely Cicer arietinum L. (chickpea), Lens culinaris L. Medikus (lentil), Cajanus cajan L. Millspaugh (pigeonpea) Pisum
sativum L. (pea). 13 RAPD primers produced 100 amplicons, out of which 98 were polymorphic. Eight primers produced
variety specific bands. SDS-PAGE analysis of seed storage proteins produced 116 bands. One protein band of 35 kD weight
was common across species and varieties. Chickpea cultivars showed maximum number of protein bands (17), while
pigeonpea recorded minimum bands (12). The molecular weight of bands ranged from 16 kD in pea to 99 kD in lentil.
Dendrogram based on the similarity coefficient index showed that grouping of genotypes in different clusters was strictly
as per the species without a mismatch and can be used as a tool for establishing phylogeny in pulse crops.
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Date 2019-03-18T08:21:27Z
2019-03-18T08:21:27Z
1001-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/17384
 
Language English
 
Relation Not Available;
 
Publisher society for agril. Biochem