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Genetic transformation of chilli ( Capsicum annuum L.) with Dreb 1A transcription factor known to impart drought tolerance

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Title Genetic transformation of chilli ( Capsicum annuum L.) with Dreb 1A transcription factor known to impart drought tolerance
Not Available
 
Creator Manamohan Maligeppagol*, R Manjula, Prakash M Navale, K Prasad Babu, Bhimanagoud M Kumbar and R H Laxman
 
Subject Transformation, chilli, drought tolerance, dreb1A
 
Description Not Available
Chilli, an important commercial crop, is recalcitrant to
in vitro
regeneration. In the present study, an attempt has been
made to optimize the
in vitro
regeneration of two chilli local cultivars,
viz.
, G4 and LCA334, and transform G4 with a
transcription factor dreb1A under the control of a desiccation inducible promoter rd29A, known to impart desiccation
tolerance, using binary vector pCAMBIA 2301. Different phytohormones and their concentrations in MS medium were
evaluated for
in vitro
response; 0.25 mg L
-1
zeatin and 2 mg L
-1
phenyl acetic acid (PAA) supported the highest regeneration
rate (37.86%). Among the explants, cotyledonary leaf exhibited a higher (51%) regeneration response compared to that of
hypocotyls (24.73%), and genotype G4 had better (33.80%) regeneration rate compared to LCA334. Rooting of the shoots
was the highest in MS medium with 2 mg L
-1
indole butyric acid (IBA) compared to other hormones. The presence of
transgene was confirmed by polymerase chain reaction (PCR) and Southern blotting. The acclimatized transformants were
grown in pots and screened for drought tolerance. Some of the transformants showed improved tolerance to drought by
lower wilting compared to the control plants. The transformation and regeneration protocol described in the present study
may help in optimization of transformation of chilli for agronomic traits of interest.
Not Available
 
Date 2019-03-19T03:38:07Z
2019-03-19T03:38:07Z
2016-01-01
 
Type Article
 
Identifier Not Available
0975-0967 (Online); 0972-5849 (Print)
http://krishi.icar.gov.in/jspui/handle/123456789/17416
 
Language English
 
Relation Not Available;
 
Publisher NISCAIR-CSIR, India