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Light intensity and temperature affect systemic spread of silencing signal in transient agro-infiltration studies

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Title Light intensity and temperature affect systemic spread of silencing signal in transient agro-infiltration studies
Not Available
 
Creator Basavaprabhu L. Patil*
Claude M. Fauquet
 
Subject RNAi
Light
Temperature
 
Description Not Available
RNA silencing is a sequence-specific post-transcriptional gene inactivation mechanism that operates in diverse organisms and that can extend beyond its site of initiation, owing to the movement of the silencing signal, called non-autonomous gene silencing. Previous studies have shown that several factors manifest the movement of the silencing signal, such as the size (21 or 24 nucleotides) of the secondary small interfering RNA (siRNA) produced, the steady-state concentration of siRNAs and their cognate messenger RNA (mRNA) or a change in the sink-source status of plant parts affecting phloem translocation. Our study shows that both light intensity and temperature have a significant impact on the systemic movement of the silencing signal in transient agroinfiltration studies in Nicotiana benthamiana. At higher light intensities (≥ 450 μE/m(2)/s) and higher temperatures (≥ 30 °C), gene silencing was localized to leaf tissue that was infiltrated, without any systemic spread. Interestingly, in these light and temperature conditions (≥ 450 μE/m(2) /s and ≥ 30 °C), the N. benthamiana plants showed recovery from the viral symptoms. However, the reduced systemic silencing and reduced viral symptom severity at higher light intensities were caused by a change in the sink-source status of the plant, ultimately affecting the phloem translocation of small RNAs or the viral genome. In contrast, at lower light intensities (
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Date 2019-03-19T06:23:31Z
2019-03-19T06:23:31Z
2015-06-01
 
Type Research Paper
 
Identifier B. L. Patil* and C. M. Fauquet (2015) Light intensity and temperature affect systemic spread of silencing signal in transient agro-infiltration studies. Molecular Plant Pathology. 16(5): 484-494.
DOI: 10.1111/mpp.12205 © 2014
http://krishi.icar.gov.in/jspui/handle/123456789/17459
 
Language English
 
Relation Not Available;
 
Publisher MOLECULAR PLANT PATHOLOGY