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Sequence diversity among badnavirus isolates infecting black pepper and related species in India

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Title Sequence diversity among badnavirus isolates infecting black pepper and related species in India
Not Available
 
Creator Bhat, A.I., Sasi, S.,. Revathy, K.A., Deeshma, K.P. and Saji, K.V.
 
Subject Piper yellow mottle virus
RT/RNaseH region sequence
Variability
Piper spp
 
Description Not Available
The badnavirus, piper yellow mottle virus
(PYMoV) is known to infect black pepper (Piper nigrum),
betelvine (P. betle) and Indian long pepper (P. longum) in
India and other parts of the world. Occurrence of PYMoV
or other badnaviruses in other species of Piper and its
variability is not reported so far. We have analysed
sequence variability in the conserved putative reverse
transcriptase (RT)/ribonuclease H (RNase H) coding region
of the virus using specific badnavirus primers from 13 virus
isolates of black pepper collected from different cultivars
and regions and one isolate each from 23 other species of
Piper. Of these, four species failed to produce expected
amplicon while amplicon from four other species showed
more similarities to plant sequences than to badnaviruses.
Of the remaining, isolates from black pepper, P. argyrophyllum,
P. attenuatum, P. barberi, P. betle, P. colubrinum,
P. galeatum, P. longum, P. ornatum, P. sarmentosum
and P. trichostachyon showed an identity of >85 % at the
nucleotide and >90 % at the amino acid level with PYMoV
indicating that they are isolates of PYMoV. On the other
hand high sequence variability of 21–43 % at nucleotide
and 17–46 % at amino acid level compared to PYMoV was
found among isolates infecting P. bababudani, P. chaba, P.
peepuloides, P. mullesua and P. thomsonii suggesting the
presence of new badnaviruses. Phylogenetic analyses showed close clustering of all PYMoV isolates that were well separated from other known badnaviruses. This is the
first report of occurrence of PYMoV in eight Piper spp and
likely occurrence of four new species in five Piper spp.
Not Available
 
Date 2019-11-30T11:22:34Z
2019-11-30T11:22:34Z
2014-07-10
 
Type Research Paper
 
Identifier Bhat, A.I., Sasi, S.,. Revathy, K.A., Deeshma, K.P. and Saji, K.V. 2014. Sequence diversity among badnavirus isolates infecting black pepper and related species in India. Virus Dis, 25:402–407.
DOI 10.1007/s13337-014-0221-0
http://krishi.icar.gov.in/jspui/handle/123456789/26057
 
Language English
 
Relation Not Available;
 
Publisher Springer