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Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum).

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Title Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum).
Not Available
 
Creator Siljo, A. and Bhat, A.I.
 
Subject RT-LAMP.
Sensitivity.
Real-time RT-PCR
Validation
 
Description Not Available
Cardamom being a perennial and propagated
vegetatively, Banana bract mosaic virus (BBrMV) in
cardamom spreads mainly through infected material.
A reverse transcription loop-mediated isothermal
amplification (RT-LAMP) assay was developed for easy
and quick detection of the virus. The following
conditions proved optimal for amplification: 4 mM of
magnesium sulphate, 1.2Mof betaine, 65 °C, and 1 h of
reaction time. The results were assessed visually by
turbidity and green fluorescence (induced by adding
manganese chloride and calcein) in the reaction tube
and also by gel electrophoresis. The assay successfully
detected the virus in infected plants whereas no crossreaction
was recorded with healthy plants. The detection
limit for RT-LAMP was up to 100 times that for
conventional RT-PCR and on a par with that for realtime
RT-PCR. The assay was validated by testing field
samples of cardamom plants from different cardamomgrowing
tracts in Kerala, India
Not Available
 
Date 2019-11-30T11:22:57Z
2019-11-30T11:22:57Z
2014-01-12
 
Type Research Paper
 
Identifier Siljo, A. and Bhat, A.I. 2014. Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum). Eur J Plant Pathol 138:209–214.
DOI 10.1007/s10658-013-0318-0
http://krishi.icar.gov.in/jspui/handle/123456789/26060
 
Language English
 
Relation Not Available;
 
Publisher Springer