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Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based Reverse Transcription-Quantitative PCR

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Title Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based Reverse Transcription-Quantitative PCR
Not Available
 
Creator Siljo, A., Bhat, A.I. and Biju, C.N
 
Subject Banana bract mosaic virus
Cardamom
Cardamom mosaic virus
Detection
RT-qPCR
Sensitivity
 
Description Not Available
Cardamom being perennial, propagated vegetatively,
detecting viruses in planting material is important
to check the spread of viruses through infected material.
Thus development of effective and sensitive assay for
detection of viruses is need of the time. In this view, assay
for the detection of Cardamom mosaic virus (CdMV) and
Banana bract mosaic virus (BBrMV), infecting cardamom
was developed using SYBR Green one step reverse transcription-
quantitative PCR (RT-qPCR). The RT-qPCR
assay amplified all isolates of CdMV and BBrMV tested
but no amplification was obtained with RNA of healthy
plants. Recombinant plasmids carrying target virus regions
corresponding to both viruses were quantified, serially
diluted and used as standards in qPCR to develop standard
curve to enable quantification. When tenfold serial dilutions
of the total RNAs from infected plants were tested
through RT-qPCR, the detection limit of the assay was
estimated to be 16 copies for CdMV and 10 copies for
BBrMV, which was approximately 1,000-fold higher than
the conventional RT-PCR. The RT-qPCR assay was validated
by testing field samples collected from different
cardamom growing regions of India. This is the first report
of RT-qPCR assay for the detection of CdMV and BBrMV
in cardamom.
Not Available
 
Date 2019-11-30T11:23:16Z
2019-11-30T11:23:16Z
2014-01
 
Type Research Paper
 
Identifier Siljo, A., Bhat, A.I. and Biju, C.N. 2014. Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based Reverse Transcription-Quantitative PCR. Virus Disease 25: 137-141.
DOI 10.1007/s13337-013-0170-z
http://krishi.icar.gov.in/jspui/handle/123456789/26061
 
Language English
 
Relation Not Available;
 
Publisher Springer