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EST linked markers in Solanum melongena responsive to abiotic stress using computational methods

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Title EST linked markers in Solanum melongena responsive to abiotic stress using computational methods
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Creator REENA ROSY THOMAS, M. K. CHANDRA PRAKASH, M. KRISHNA REDDY, RIAZ MAHMOOD AND PAPIYA MONDAL
 
Subject Abiotic stress, Computational methods, EST sequences, Markers, Solanum melongena
 
Description Not Available
Plants are exposed to a wide range of stresses and they respond to these stressed conditions by the activation of stress responsive genes. Microsatellites occur at thousands of locations in expressed regions, additionally, they have a higher mutation rate than other areas of DNA leading to genetic markers. A computational method was developed to capture exact repeat motifs in EST sequences of Solanum
melongena and extract the sequences. This mathematical algorithm based repeat detection program searches for perfect repeats in the EST sequences without the need to specify the pre-defined repeat pattern for di, tri, tetra-, upto octa repeat with the exception of mono nucleotides. Hexa repeat motifs were abundantly
available followed by tetra and di repeats. The identified EST derived markers with repeat motif can be used to identify the precise inheritance pattern of the expressed region. The redundant ESTs were filtered and 121
unique ESTs in S. melongena were found to have potential markers. These were spanned across 19 major proteins superfamilies having conserved domains associated with abiotic stress tolerance. These robust and potential EST derived markers are linked to genes that encode for specific traits providing tolerance against abiotic stresses which is useful in marker-assisted breeding programs.
Not Available
 
Date 2019-03-20T06:32:50Z
2019-03-20T06:32:50Z
2017
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17529
 
Language English
 
Relation Not Available;
 
Publisher National Environmentalists Association