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Molecular Markers Associated to Two Non-allelic Genic Male Sterility Genes in Peppers (Capsicum annuum L.)

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Title Molecular Markers Associated to Two Non-allelic Genic Male Sterility Genes in Peppers (Capsicum annuum L.)
Not Available
 
Creator Not AvailablePonnam Naresh1, Shih-wen Lin, Chen-yu Lin, Yen-wei Wang, Roland Schafleitner, Andrzej Kilian and Sanjeet Kumar
 
Subject genic male sterility, genotyping by sequencing, hot pepper, hybrid seed production, SNP, sweet pepper
 
Description Not Available
Male sterility is of high importance in hybrid seed production of hot and sweet
peppers. Genic (or nuclear) male sterility (GMS) is a simply inherited (usually monogenic
recessive) and highly stable trait. However, one major disadvantage of using GMS is
1:1 segregation of male sterile to male fertile plants in every subsequent generation.
Molecular markers tightly linked to genic male sterility (ms) genes would facilitate an
efficient and rapid transfer of ms genes into different genetic backgrounds through
marker-assisted backcrossing. The two non-allelic genic male sterility genes ms3 and
msw in hot and sweet pepper backgrounds, respectively, are monogenic recessive.
Genotyping by sequencing (GBS) in an F2 population segregating for ms3 gene in
hot pepper and in an F6 inbred near-isogenic line (NIL) population segregating for
msw gene in sweet pepper yielded 9,713 and 7,453 single nucleotide polymorphism
markers, respectively. Four candidate SNPs co-segregating with ms3 gene and one cosegregating
with msw gene were identified by bulk segregant analysis and physically
mapped to chromosomes 1 and 5, respectively. In hot pepper, two markers [HPGMS2
(CAPS) and HPGMS3 (dCAPS)] located 3.83 cM away from the ms3 gene and in sweet
pepper the dCAPS marker SPGMS1 co-segregated (completely linked) with the msw
gene were developed. These markers will increase the efficacy of the male sterility genes
for pepper breeding, as they can be useful in developing the genic male sterile lines
in parental inbred lines of commercial hybrids through marker-assisted backcrossing,
hybrid seed production, and genetic purity testing of hybrid seeds.
Not Available
 
Date 2019-03-29T03:40:00Z
2019-03-29T03:40:00Z
2018-10-16
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17743
 
Language English
 
Relation Not Available;
 
Publisher Frontiers in Plant Science