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Molecular identification and biological characterization of a begomovirus associated with okra enation leaf curl disease in India

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Title Molecular identification and biological characterization of a begomovirus associated with okra enation leaf curl disease in India
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Creator Venkataravanappa, V., Lakshminarayana Reddy, C.N., Salil Jalali, Rob W. Briddon and Krishna Reddy, M
 
Subject Okra enation leaf curl virus .Whitefly . Bemisia tabaci . Recombination . Begomovirus
 
Description Not Available
An unusual symptom was noted in okra originating
from Haryana state (India) consisting of leaf curl
associated with enations. These were distinct from the leaf
curl and/or vein yellowing symptoms usually shown by
okra. PCR-mediated amplification was used to show the
presence of a begomovirus and the complete genome
sequences were determined for seven isolates. The sequences
showed high levels of nucleotide sequence identity
(91.9–99.1 %). In comparison to begomovirus sequences
available in the databases the okra sequences
showed the highest levels of nucleotide sequence identity
(84.5 to 87.1 %) with Mesta yellow vein mosaic virus
(MeYVV) and thus were classified as belonging to a
novel begomovirus species, tentatively named Okra enation
leaf curl virus. In common with the majority of
begomoviruses, Okra enation leaf curl virus (OELCuV)
was shown to have a recombinant origin. Analysis of the
transmission characteristics of a wild-type virus isolate by
Bemisia tabaci showed the minimum acquisition access
period to be 1 h and the minimum inoculation access
period to be 30 min, with female insects transmitting with
a greater efficiency than male insects. Under controlled
conditions the host range of the virus was shown to be
very narrow, limited to two species in the family
Malvaceae, okra (Abelmoschus esculentus) and hollyhock
(Althaea rosea), and seven in the family Solanaceae.
Not Available
 
Date 2019-03-29T07:44:33Z
2019-03-29T07:44:33Z
2014-01-01
 
Type Journal
 
Identifier 10
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17773
 
Language English
 
Relation Not Available;
 
Publisher Not Available