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Molecular Approach Coupled with Biochemical Attributesto Elucidate the Presence of DYMV in Leaf Samplesof Lablab purpureus. L Genotypes

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Title Molecular Approach Coupled with Biochemical Attributesto Elucidate the Presence of DYMV in Leaf Samplesof Lablab purpureus. L Genotypes
Not Available
 
Creator Nagendra Rai1, Krishna Kumar Rai, V. Venkataravanappa, Sujoy Saha
 
Subject Dolichos yellowmosaic virus (DYMV) . Lablab perpureus . Antioxidant enzymes . Photosynthetic pigments
 
Description Not Available
A laboratory study was delineated to ascertain the impact and the extent of Dolichos
yellow mosaic virus (DYMV) on biochemical constituents and various enzyme levels in the
leaves of hyacinth bean. DYMV-infected leaves of all the genotypes used in the study revealed
significant and consistent changes in activities of CAT, APX, PPO, DHAR, and MDHAR
paralleled with a compelling hike in proline levels. Unlike that in non-infected leaves of the
genotypes VRSEM-301 and VRSEM-749, VRSEM-894 and VRSEM-855, the enzyme level
did not alter much which extended equally with increased phenolics, suggesting a wellcoordinated
generation of free radicals thereby suppressing oxidative stress in the latter. The
genotypes were also evaluated at molecular level for elucidating the presence of the virus by
using five sets of primer pairs. Two primers viz., DAC1 and DAC2 witnessed the presence of
the virus in both non-infected and infected leaves. The difference in the appearance and/or
disappearance of bands according to non-infected to infect reverberates the variation between
genotypes in defense against infection.
Not Available
 
Date 2019-03-29T08:06:51Z
2019-03-29T08:06:51Z
2016-01-01
 
Type Journal
 
Identifier 8
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/17787
 
Language English
 
Relation Not Available;
 
Publisher Not Available