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Characterization of Begomoviruses associated with Papaya Leaf Curl Disease in India

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Title Characterization of Begomoviruses associated with Papaya Leaf Curl Disease in India
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Creator Krishna Reddy, M., Venkataravanappa, V., Madhuvanthi, B. and Jalali, S
 
Subject Papaya leaf curl virus, detection, molecular characterization, Begomovirus, PCR, amplification, papaya
 
Description Not Available
For molecular characterization of papaya leaf curl causing begomoviruses,
papaya samples showing symptoms of leaf curl, vein clearing, enations and reduced
leaf size were collected from Kodur of Andhra Pradesh, Bangalore of Karnataka,
Coimbatore of Tamil Nadu, Lucknow of Uttar Pradesh and Delhi States of India.
Using primers specific to begomovirus DNA-A used the total DNA isolated from
these samples for begomovirus infection. For molecular differentiation of papaya
leaf curl isolates, cloning and sequencing of PCR amplified fragments was done. The
complete DNA-A sequence of four papaya leaf curl isolates was characterized.
Comparative sequence analysis with 21 other begomoviruses has indicated that the
PaLC. Coimbatore isolate has 77.5% nucleotide identity with Ageratum enation virus
(AEV), PaLCV.Kodur has 90.3% nucleotide identity with Croton yellow vein mosaic
virus (CrYVMV), PaLC.UP13 isolate has 94.7% nucleotide identity with Papaya leaf
curl virus (PaLCV) and PaLC.New Delhi isolate has 95.5% nucleotide identity with
Tomato leaf curl New Delhi virus (ToLCNDV). Phylogenetic analysis and nucleotide
comparison has indicated that in India, papaya is infected with more than three
distinct begomoviruses. PaLC.Coimbatore isolate has nucleotide identity less than
89% with another known begomoviruses and named as Papaya leaf curl Coimbatore
virus (PaLCCoV) and considered as a new begomovirus infecting papaya in India
Not Available
 
Date 2019-03-30T06:52:06Z
2019-03-30T06:52:06Z
2010-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/17820
 
Language English
 
Relation Not Available;
 
Publisher Not Available