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Begomovirus characterization and development of phenotypic and DNA-based diagnostics for screening of okra genotyperesistance against Bhendi yellow vein mosaic virus

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Title Begomovirus characterization and development of phenotypic and DNA-based diagnostics for screening of okra genotyperesistance against Bhendi yellow vein mosaic virus
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Creator Venkataravanappa V., C.N. Lakshminarayana Reddy and M. Krishna Reddy
 
Subject Bhendi yellow vein mosaic virus (BYVMV) Okra yellow vein mosaic virus (OYVMV) Begomovirus Genotypes PCR Susceptibility Varieties Resistance
 
Description Not Available
The leaf sample from okra plants showing the
yellow vein mosaic disease symptoms was collected in
Karnataka state, India. The genome of the virus was
amplified, cloned and sequenced. Sequence analysis
revealed that the viral genome (GU112065) is 2,741 bp in
length and genome is similar to that of monopartite begomoviruses
originating from the Old World, with seven
conserved ORFs. Further nucleotide (nts) sequence comparisons
showed that the genome has the highest sequence
identities of 96.1 % with Bhendi yellow vein mosaic virus
(BYVMV) (GU112057) and 89.7 % with okra yellow vein
mosaic virus (OYVMV) (AJ002451) infecting okra in India
and Indian subcontinent. These results suggested that the
isolate is a new strain of BYVMV. To identify the resistance
source to BYVMV, the okra genotypes were screened under
both artificial and natural conditions. None of the genotypes
showed immunity to the disease. However, the genotypes
Nun 1145 and Nun 1144 showed moderate resistance and
genotypes M10, Nun 1142, Nun 1140 showed moderately
susceptible reactions under both glass house and field
conditions. Further, dot-blot hybridization using nonradioactive
(digoxigenin) DNA probe showed that the virus was
also detected in the symptomless plants.
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Date 2019-03-30T06:48:23Z
2019-03-30T06:48:23Z
2013-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/17818
 
Language English
 
Relation Not Available;
 
Publisher Not Available