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Comparative transmission of Bhendi yellow vein mosaic virus by two cryptic species of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

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Title Comparative transmission of Bhendi yellow vein mosaic virus by two cryptic species of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
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Creator Venkataravanappa V., Kodandaram M. H, Lakshminarayana Reddy C N, Shankarappa K S, Krishna Reddy M
 
Subject Bhendi yellow vein mosaic virus Bemisia tabaci Genetic species Transmission
 
Description Not Available
The leaf sample from okra plants showing
prominent yellow vein mosaic symptoms and healthy plant
without any virus symptoms were collected from farmer’s
field. The presence of begomovirus in the infected sample
was confirmed by polymerase chain reaction (PCR) and the
amplicons were cloned and sequenced. The genome analysis
showed that the isolate in the present study had 99%
nucleotide identity with Bhendi yellow vein mosaic virus
(BYVMV) revealing it as BYVMV variant. The genetic
species of Bemisia tabaci collected from fields were
identified as Asia-1 and MEAM-1 genetic species based on
silver leaf assay, sequence characterized amplified region
marker, and mtCOI gene sequence. The comparative virus–
vector relationship of both genetic species of B. tabaci
indicates a minimum of two and three B. tabaci in MEAM-
1 and Asia-1 genetic species, respectively, per plant were
required to transmit the disease. The minimum acquisition
access period and inoculation access period of 15 (MEAM-
1) and 20 min (Asia-1) were required to transmit the
YVMD; it was further confirmed by nucleic acid
hybridization using coat protein gene-specific probe of
BYVMV. With respect to the sex, the female B. tabaci
were more efficient in transmitting the disease as compared
to male ones in both the genetic species of B. tabaci. The
MEAM-1 to transmit the BYVMV more efficiently than
Asia-1 genetic species of B. tabaci.
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Date 2019-03-30T06:47:54Z
2019-03-30T06:47:54Z
2018-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/17817
 
Language English
 
Relation Not Available;
 
Publisher Not Available