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Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India

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Title Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India
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Creator Venkataravanappa V., Swarnalatha P., Saha S, Lakshminarayana Reddy C N., Krishna Reddy M
 
Subject Tomato Phytoplasma India Pea nut witches broom disease 16SrII Polymerase reaction (PCR)
 
Description Not Available
Tomato plants showing witches broom symptoms
were collected from different states of India. The
presence of phytoplasma infection was confirmed by PCR
using phytoplasma-specific primer of 16S rRNA and SecY
gene. The sequence analysis of 16S rRNA and SecY gene
of eight tomato big bud phytoplasmas showed maximum
nucleotide (nt) identity of 95 - 100% with Peanut WB group
(16SrII). Further in-silico RFLP analysis of 16S rRNA
gene of TBB-Pun1, TBB-Ban, TBB-mal, TBB-Guj and
TBB-Vns showed similarity coefficient of 0.68–0.95. Since
threshold similarity coefficient for classifying the phytoplasma
into new subgroup is set at 0.97, the strain under
study significantly distinct from the representative strains
in the subgroups of pea nut witches broom. Further, the
phylogenetic analysis of tomato big bud phytoplasmas
revealed that, they are closely clustered with peanut witches’-
broom strains (16Sr II), specifically within the 16Sr
II-D and 16Sr II-A subgroups. A comprehensive recombination
analysis showed the evidence of both intra and
inter-species recombination in seven tomato big bud isolates
with most part of their 16Sr RNA F2nR2 fragments
descending from Ca.P.brasiliense (16Sr XV) as major
parent, except isolate TBB-Vns which had an intra species
recombination with Cactus witches-broom-16Sr II-L as
major parent. Similarly, in case of SecY gene, all the seven
isolates have intra-species recombination with major portion
descending from Vinca virescence - [16Sr VI - A] and
Potato purple top wilt - [16Sr XVIII - B]. The genetic similarities
and the potential threat of this new phytoplasma
belonging to 16Sr II group of Peanut witches’ broom’
group infecting tomato in India are discussed.
Not Available
 
Date 2019-04-25T06:39:24Z
2019-04-25T06:39:24Z
2017-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/18877
 
Language English
 
Relation Not Available;
 
Publisher Not Available