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Identification of novel microsatellite markers in okra (Abelmoschus esculentus (L.) Moench) through next-generation sequencing and their utilization in analysis of genetic relatedness studies and cross-species transferability

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Title Identification of novel microsatellite markers in okra (Abelmoschus esculentus (L.) Moench) through next-generation sequencing and their utilization in analysis of genetic relatedness studies and cross-species transferability
 
Creator KUNDAPURA V. RAVISHANKAR
GAYATHRI MUTHAIAH
PITCHAIMUTHU MOTTAIYAN
and SANTHOSH-KUMAR GUNDALE
 
Subject microsatellite markers; transferability; polymorphic information content; Abelmaschus esculentus.
 
Description Not Available
Abelmoschus esculentus L. (Malvaceae), populary known
as okra, is one of the important pod vegetable crops
in tropics and subtropics. To develop the microsatellite
markers, we have partially sequenced genomicDNA using
Roche 454 Titanium pyrosequencing technology. A total
of 979,806 bp data from 61,722 reads were obtained.
These reads were assembled into 3735 contigs of which
2708 had microsatellites. Primers were designed for 402
microsatellites, from which 50 randomly selected SSR
primerswere standardized for amplification of okraDNA.
Genetic analysis was done by employing 29 genotypes.
Polymorphic information content (PIC) across all 50 loci
ranged from 0.000 to 0.865 with a mean value of 0.519.
The observed and expected heterozygosity ranged from
0.000 to 0.750 and 0.000 to 0.972, respectively. Alleles
per locus ranged from 1 to 27. This is the first report
on the development of genomic SSR markers in okra
using next-generation sequencing technology. We have
also assessed cross species transferability of microsatellite
markers with related wild species A. tetraphyllus (84%), A.
ficulneus (78%), A. moschatus (92%), A. angulosus grandiflorus
(90%) and A. caillei (86%), and demonstrated their
use in genetic relatedness study and molecular characterization
Not Available
 
Date 2019-04-24T08:03:53Z
2019-04-24T08:03:53Z
2018-04-11
 
Type Research Paper
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/18818
 
Language English
 
Publisher Indian Academy of Sciences