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In vitro plantlet regeneration study for in vitro conservation in Curculigo orchioides - an endangered medicinal plant

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Title In vitro plantlet regeneration study for in vitro conservation in Curculigo orchioides - an endangered medicinal plant
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Creator P.E. Rajasekharan, S. Ganeshan and Sunitha Bhaskaran
 
Subject Endangered, Extra vitrum, In vitro conservation
 
Description Not Available
Abstract
In vitro studies on Curculigo orchioides Gaertn. (Hypoxidaceae) was undertaken to devise a protocol for in vitro conservation of this endangered species. Fresh underground tubers with shoot apices were used to initiate cultures. Later the in vitro derived leaves and roots were used as explants on MS medium supplemented with 8.87μM BA, to obtain multiple shoots. Callus phase was avoided to maintain true-to type cultures and direct encourage regeneration. Simultaneous rooting enabled successful establishment extra vitrum, with 100% survival rates. In vitro conservation was accomplished at 100 C and cultures could be maintained for a minimum period of 1 year without an intermediate subculture.
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Date 2019-05-22T07:10:45Z
2019-05-22T07:10:45Z
2016-10-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19797
 
Language English
 
Relation Not Available;
 
Publisher Not Available