Record Details

Genetic diversity analysis and chemical profiling of Indian Acorus calamus accessions from South and North-East India

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Genetic diversity analysis and chemical profiling of Indian Acorus calamus accessions from South and North-East India
Not Available
 
Creator M N Mythili Avadhani1, C Immanuel Selvaraj , P E Rajasekharan, V K Rao, H Munirajappa and C Tharachand
 
Subject β-Asarone, HPLC, medicinal plant, ploidy, RAPD
 
Description Not Available
Acorus calamus L. (Family: Acoraceae) is a well-known traditional, endangered, medicinal and aromatic plant mainly found in India and China. The plant is also widely used in industrial, pharmaceutical and food industries. In the present study, 20 different accessions of Indian A. calamus were subjected to the study of genetic diversity (RAPD), and cytogenetic and phytochemical (β-asarone) analysis. For RAPD analysis, 9 primers were chosen which generated 107 DNA fragments. The average percentage of polymorphism was recorded to be 67.23%. The primer OPA 12 showed the highest (100%) polymorphism, whereas the lowest (38.2%) polymorphism was observed for the primer OPBB 6. The polymorphism information content (PIC) values ranged 0.44 (OPA 7) to 0.18 (OPA11), while marker index (MI) values ranged 4.74 (OPA 7) to 0.36 (OPA 11). A dendogram was constructed by UPGMA method and the robustness of the tree was confirmed by bootstrap analysis with 1000 pseudo samples. For cytogenetic analysis, the 20 A. calamus accessions were screened for their ploidy status. The accessions were found to be either diploid or triploids. The phytochemical analysis of β-asarone content was determined through by HPLC method. The β-asarone concentration varied in the range of 2.2 to 7.2 mg/100 mg. The results of present study indicated the presence of low level of polymorphism among the A. calamus accessions of South India and North-East India. The phytochemical and cytogenetic analysis revealed that both diploid and triploid have low concentration of β-asarone irrespective of their geographical location
Not Available
 
Date 2019-05-25T07:26:43Z
2019-05-25T07:26:43Z
2015-09-21
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/19860
 
Language English
 
Relation Not Available;
 
Publisher Not Available