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High resolution LC-MS characterization of phenolic compounds and the evaluation of antioxidant properties of a tropical purple radish genotype

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Title High resolution LC-MS characterization of phenolic compounds and the evaluation of antioxidant properties of a tropical purple radish genotype
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Creator Tanmay Kumar Koley, Zareen Khan, Dasharath Oulkar, B.K. Singh, Arti Maurya, B. Singh, Kaushik Banerjee
 
Subject Radish (Raphanus sativus L., Brassicaceae); Metabolic profiling; Phenolics; Anthocyanins; Flavonols; Antioxidant assays; High resolution LC-MS
 
Description Not Available
This study reports qualitative profiling of the phenolic compounds in an indigenously
developed purple radish genotype VRRAD-151 using ultra performance liquid chromatography
with quadrupole time of flight mass spectrometry. The root and leaf samples were harvested at
the horticultural maturity stage of the genotype. Roots were divided into the periderm, and xylem,
and the leaf samples were divided into petiole, and lamina, and these were separately extracted with
methanol before the LC-MS analysis. A total of 66 compounds, including 23 flavonols, 1 dihydroflavonols,
4 flavonones, 4 flavones, 28 anthocyanins, 2 isoflavonoids, 3 phenolic acids, and 1
hydroxybenzaldehyde were putatively identified based on high resolution accurate mass analysis
with the data processing through UNIFI , which is a comprehensive compound identification software
solution. An in-house developed database comprising the secondary metabolites of polyphenols
was used for the screening purpose, and each phenolic compound was identified based on the
detection of the precursor ion, and at least one characteristic fragment ion, each with less than 5
ppm of mass error. Anthocyanins were the most abundant type of phenolics exhibiting 59% in leaf
petiole, 80% in root periderm, and 90% in root xylem. The relative concentration of anthocyanins
was lower (11%) in the leaf lamina. Cyanidins were the most predominant anthocyanins accounting
for 54, 100, 90 and 65%, in leaf lamina, leaf petiole, root periderm and root xylem, respectively.
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Date 2019-07-26T10:39:39Z
2019-07-26T10:39:39Z
2017-12-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/21812
 
Language English
 
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Publisher Not Available