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Molecular diversity, recombination and population structure of alphasatellites associated with begomovirus disease complexes

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Title Molecular diversity, recombination and population structure of alphasatellites associated with begomovirus disease complexes
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Creator RV Kumar, D Singh, AK Singh, S. Chakraborty
 
Subject Begomovirus. Alphasatellites, Plant virus evolution, Recombination, Genetic variability, Pathogenesis
 
Description Not Available
The genus, begomovirus (family Geminiviridae) includes a large number of viruses infecting awide range of plant
species worldwide. The majority of monopartite begomoviruses are associated with satellites (betasatellites)
and/or satellite-like molecules (alphasatellites). In spite of the Indo-China region being regarded as the centre
of origin of begomoviruses and satellites, a detailed study on the emergence and evolution of alphasatellites in
India has not yet conducted. Our present analysis indicated the association of 22 alphasatelliteswith monopartite
and bipartite begomovirus-betasatellite complexes in India. Based on sequence pairwise identity, these
alphasatellites were categorized into five distinct groups: Cotton leaf curl alphasatellite, Gossypium darwinii
symptomless alphasatellite, Gossypium mustelinum symptomless alphasatellite, Okra leaf curl alphasatellite
and an unreported Chilli leaf curl alphasatellite (ChiLCA). Furthermore, infectivity analysis of the cloned ChiLCA
along with the viral components of either cognate or non-cognate chilli-infecting begomoviruses on Nicotiana
benthamiana suggested that ChiLCA is dispensable for leaf curl disease development. It is noteworthy that in
the presence of ChiLCA, a marginal decrease in betasatellite DNA level was noticed. Additionally, high genetic
variability and diverse recombination patterns were detected among these alphasatellites, and the nucleotide
substitution rate for the Rep gene of ChiLCA was determined to be 2.25 × 10−3 nucleotides/site/year. This
study highlights the genetic distribution, and likely contribution of recombination and nucleotide diversity in
facilitating the emergence of alphasatellites.
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Date 2019-10-22T04:56:19Z
2019-10-22T04:56:19Z
2017-01-01
 
Type Research Paper
 
Identifier 16
1567-1348
http://krishi.icar.gov.in/jspui/handle/123456789/23795
 
Language English
 
Relation Not Available;
 
Publisher Elsevier